1 Ocak 2019 Dergi makalesi Açık Erişim

Ertekin, Oezlem; Kaymak, Tugrul; Pirincci, Serife Seyda; Akcael, Esin; Ozturk, Selma

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{
  "@context": "https://schema.org/", 
  "@id": 90731, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Ertekin, Oezlem"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Republ Turkey Minist Food Agr & Livestock, Gen Directorate Food & Control, Ankara, Turkey", 
      "name": "Kaymak, Tugrul"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Sci & Technol Res Council Turkey, TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Kocaeli, Turkey", 
      "name": "Pirincci, Serife Seyda"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Sci & Technol Res Council Turkey, TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Kocaeli, Turkey", 
      "name": "Akcael, Esin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Sci & Technol Res Council Turkey, TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Kocaeli, Turkey", 
      "name": "Ozturk, Selma"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "Antibodies are the basic components of immuno-analytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins. The designed scheme puts emphasis on solvent-and matrix-related characterization steps and was used to comparatively evaluate eight monoclonal antibodies. The selected antibody was tolerant to 40% methanol, 20% acetonitrile, 30% acetone and 40% ethanol and did not interact with corn, red pepper or hazelnut extracts. Immunoaffinity columns developed with the selected antibody were validated by 15 independent aflatoxin analysis laboratories.", 
  "headline": "Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development", 
  "identifier": 90731, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development", 
  "url": "https://aperta.ulakbim.gov.tr/record/90731"
}