Dergi makalesi Açık Erişim

Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development

   Ertekin, Oezlem; Kaymak, Tugrul; Pirincci, Serife Seyda; Akcael, Esin; Ozturk, Selma

Antibodies are the basic components of immuno-analytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins. The designed scheme puts emphasis on solvent-and matrix-related characterization steps and was used to comparatively evaluate eight monoclonal antibodies. The selected antibody was tolerant to 40% methanol, 20% acetonitrile, 30% acetone and 40% ethanol and did not interact with corn, red pepper or hazelnut extracts. Immunoaffinity columns developed with the selected antibody were validated by 15 independent aflatoxin analysis laboratories.

Dosyalar (189 Bytes)
Dosya adı Boyutu
bib-ab6f4bf5-e26f-4d27-bca0-408d7d8624d9.txt
md5:a25d29a1004199148f3b45a3408ab97b
189 Bytes İndir
45
9
görüntülenme
indirilme
Görüntülenme 45
İndirme 9
Veri hacmi 1.7 kB
Tekil görüntülenme 45
Tekil indirme 8
Atıflar
  • Citation Indexes: 7
Okunma İstatistikleri
  • Readers: 11

Alıntı yap