1 Ocak 2015 Dergi makalesi Açık Erişim

Eksin, Ece; Erdem, Arzum

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Eksin, Ece</dc:creator>
  <dc:creator>Erdem, Arzum</dc:creator>
  <dc:date>2015-01-01</dc:date>
  <dc:description>Homocysteine-thiolactone (HTL) is known as an intramolecular thioester of homocysteine (Hcy). The thioester chemistry of Hcy-thiolactone underlies its ability to form isopeptide bonds with the protein lysine residues, which may impair, or alter the function of protein. HTL modification is a unique post-translational protein modification that is recognized as an emergent biomarker for cardiovascular and neurovascular disease. Electrochemical detection of bovine serum albumin (BSA) before and after N-homocysteinylation was performed using a disposable graphite electrode in combination with a differential pulse voltammetry (DPV) technique. Accordingly, an enhanced detection of BSA was obtained based on the changes at the oxidation signal of BSA after N-homocysteinylation. A lower detection limit was obtained for N-homocysteinylated BSA (N-Hcy-BSA) as 1.09 mu g mL(-1) in comparison to the one of non-homocysteinylated BSA (i.e., 1.55 mu g mL(-1)). The DL of BSA and N-Hcy-BSA in fetal bovine serum medium was also calculated and found to be 3.29 mu g mL(-1) and 2.72 mu g mL(-1), respectively.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/77337</dc:identifier>
  <dc:identifier>oai:zenodo.org:77337</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>RSC ADVANCES 5(7) 4774-4779</dc:source>
  <dc:title>Electrochemical detection of N-homocysteinylated BSA in the fetal bovine serum medium</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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