1 Ocak 2015 Dergi makalesi Açık Erişim

Eksin, Ece; Erdem, Arzum

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/77337</identifier>
  <creators>
    <creator>
      <creatorName>Eksin, Ece</creatorName>
      <givenName>Ece</givenName>
      <familyName>Eksin</familyName>
      <affiliation>Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erdem, Arzum</creatorName>
      <givenName>Arzum</givenName>
      <familyName>Erdem</familyName>
      <affiliation>Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Electrochemical Detection Of N-Homocysteinylated Bsa In The Fetal Bovine Serum Medium</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2015</publicationYear>
  <dates>
    <date dateType="Issued">2015-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/77337</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1039/c4ra13303j</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">Homocysteine-thiolactone (HTL) is known as an intramolecular thioester of homocysteine (Hcy). The thioester chemistry of Hcy-thiolactone underlies its ability to form isopeptide bonds with the protein lysine residues, which may impair, or alter the function of protein. HTL modification is a unique post-translational protein modification that is recognized as an emergent biomarker for cardiovascular and neurovascular disease. Electrochemical detection of bovine serum albumin (BSA) before and after N-homocysteinylation was performed using a disposable graphite electrode in combination with a differential pulse voltammetry (DPV) technique. Accordingly, an enhanced detection of BSA was obtained based on the changes at the oxidation signal of BSA after N-homocysteinylation. A lower detection limit was obtained for N-homocysteinylated BSA (N-Hcy-BSA) as 1.09 mu g mL(-1) in comparison to the one of non-homocysteinylated BSA (i.e., 1.55 mu g mL(-1)). The DL of BSA and N-Hcy-BSA in fetal bovine serum medium was also calculated and found to be 3.29 mu g mL(-1) and 2.72 mu g mL(-1), respectively.</description>
  </descriptions>
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