1 Ocak 2019 Dergi makalesi Açık Erişim

Akbari, Soheil; Sevinc, Gulben Gurhan; Ersoy, Nevin; Basak, Onur; Kaplan, Kubra; Sevinc, Kenan; Ozel, Erkin; Sengun, Berke; Enustun, Eray; Ozcimen, Burcu; Bagriyanik, Alper; Arslan, Nur; Onder, Tamer Tevfik; Erdal, Esra

Dublin Core

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  <dc:creator>Akbari, Soheil</dc:creator>
  <dc:creator>Sevinc, Gulben Gurhan</dc:creator>
  <dc:creator>Ersoy, Nevin</dc:creator>
  <dc:creator>Basak, Onur</dc:creator>
  <dc:creator>Kaplan, Kubra</dc:creator>
  <dc:creator>Sevinc, Kenan</dc:creator>
  <dc:creator>Ozel, Erkin</dc:creator>
  <dc:creator>Sengun, Berke</dc:creator>
  <dc:creator>Enustun, Eray</dc:creator>
  <dc:creator>Ozcimen, Burcu</dc:creator>
  <dc:creator>Bagriyanik, Alper</dc:creator>
  <dc:creator>Arslan, Nur</dc:creator>
  <dc:creator>Onder, Tamer Tevfik</dc:creator>
  <dc:creator>Erdal, Esra</dc:creator>
  <dc:date>2019-01-01</dc:date>
  <dc:description>Organoid technologies have become a powerful emerging tool to model liver diseases, for drug screening, and for personalized treatments. These applications are, however, limited in their capacity to generate functional hepatocytes in a reproducible and efficient manner. Here, we generated and characterized the hepatic organoid (eHEPO) culture system using human induced pluripotent stem cell (iPSC)-derived EpCAM-positive endodermal cells as an intermediate. eHEPOs can be produced within 2 weeks and expanded long term (&gt;16 months) without any loss of differentiation capacity to mature hepatocytes. Starting from patient-specific iPSCs, we modeled citrullinemia type 1, a urea cycle disorder caused by mutations in the argininosuccinate synthetase (ASST) enzyme. The disease-related ammonia accumulation phenotype in eHEPOs could be reversed by the overexpression of the wild-type ASS1 gene, which also indicated that this model is amenable to genetic manipulation. Thus, eHEPOs are excellent unlimited cell sources to generate functional hepatic organoids in a fast and efficient manner.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/75351</dc:identifier>
  <dc:identifier>oai:zenodo.org:75351</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>STEM CELL REPORTS 13(4) 627-641</dc:source>
  <dc:title>Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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