1 Ocak 2019 Dergi makalesi Açık Erişim

Akbari, Soheil; Sevinc, Gulben Gurhan; Ersoy, Nevin; Basak, Onur; Kaplan, Kubra; Sevinc, Kenan; Ozel, Erkin; Sengun, Berke; Enustun, Eray; Ozcimen, Burcu; Bagriyanik, Alper; Arslan, Nur; Onder, Tamer Tevfik; Erdal, Esra

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{
  "@context": "https://schema.org/", 
  "@id": 75351, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Akbari, Soheil"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Sevinc, Gulben Gurhan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dokuz Eylul Univ, Hlth Sci Inst, Dept Histol & Embryol, TR-35340 Izmir, Turkey", 
      "name": "Ersoy, Nevin"
    }, 
    {
      "@type": "Person", 
      "name": "Basak, Onur"
    }, 
    {
      "@type": "Person", 
      "name": "Kaplan, Kubra"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Sevinc, Kenan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Ozel, Erkin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Sengun, Berke"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Enustun, Eray"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Ozcimen, Burcu"
    }, 
    {
      "@type": "Person", 
      "name": "Bagriyanik, Alper"
    }, 
    {
      "@type": "Person", 
      "name": "Arslan, Nur"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Koc Univ, Fac Med, TR-34450 Istanbul, Turkey", 
      "name": "Onder, Tamer Tevfik"
    }, 
    {
      "@type": "Person", 
      "name": "Erdal, Esra"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "Organoid technologies have become a powerful emerging tool to model liver diseases, for drug screening, and for personalized treatments. These applications are, however, limited in their capacity to generate functional hepatocytes in a reproducible and efficient manner. Here, we generated and characterized the hepatic organoid (eHEPO) culture system using human induced pluripotent stem cell (iPSC)-derived EpCAM-positive endodermal cells as an intermediate. eHEPOs can be produced within 2 weeks and expanded long term (>16 months) without any loss of differentiation capacity to mature hepatocytes. Starting from patient-specific iPSCs, we modeled citrullinemia type 1, a urea cycle disorder caused by mutations in the argininosuccinate synthetase (ASST) enzyme. The disease-related ammonia accumulation phenotype in eHEPOs could be reversed by the overexpression of the wild-type ASS1 gene, which also indicated that this model is amenable to genetic manipulation. Thus, eHEPOs are excellent unlimited cell sources to generate functional hepatic organoids in a fast and efficient manner.", 
  "headline": "Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling", 
  "identifier": 75351, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling", 
  "url": "https://aperta.ulakbim.gov.tr/record/75351"
}