1 Ocak 2019 Dergi makalesi Açık Erişim

Farzani, Touraj Aligholipour; Foldes, Katalin; Hanifehnezhad, Alireza; Ilce, Burcu Yener; Dagalp, Seval Bilge; Khiabani, Neda Amirzadeh; Erguenay, Koray; Alkan, Feray; Karaoglu, Taner; Bodur, Hurrem; Ozkul, Aykut

DataCite XML

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/71641</identifier>
  <creators>
    <creator>
      <creatorName>Farzani, Touraj Aligholipour</creatorName>
      <givenName>Touraj Aligholipour</givenName>
      <familyName>Farzani</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Foldes, Katalin</creatorName>
      <givenName>Katalin</givenName>
      <familyName>Foldes</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Hanifehnezhad, Alireza</creatorName>
      <givenName>Alireza</givenName>
      <familyName>Hanifehnezhad</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ilce, Burcu Yener</creatorName>
      <givenName>Burcu Yener</givenName>
      <familyName>Ilce</familyName>
      <affiliation>Ankara Univ, Biotechnol Inst, TR-06560 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Dagalp, Seval Bilge</creatorName>
      <givenName>Seval Bilge</givenName>
      <familyName>Dagalp</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Khiabani, Neda Amirzadeh</creatorName>
      <givenName>Neda Amirzadeh</givenName>
      <familyName>Khiabani</familyName>
      <affiliation>Ankara Univ, Biotechnol Inst, TR-06560 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erguenay, Koray</creatorName>
      <givenName>Koray</givenName>
      <familyName>Erguenay</familyName>
      <affiliation>Hacettepe Univ, Dept Med Microbiol, Virol Unit, Fac Med, TR-06100 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Alkan, Feray</creatorName>
      <givenName>Feray</givenName>
      <familyName>Alkan</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karaoglu, Taner</creatorName>
      <givenName>Taner</givenName>
      <familyName>Karaoglu</familyName>
      <affiliation>Ankara Univ, Virol Dept, Fac Vet Med, TR-06110 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Bodur, Hurrem</creatorName>
      <givenName>Hurrem</givenName>
      <familyName>Bodur</familyName>
      <affiliation>Saglik Bilimleri Univ, Numune Training &amp; Res Hosp, Infect Dis Clin, TR-06100 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ozkul, Aykut</creatorName>
      <givenName>Aykut</givenName>
      <familyName>Ozkul</familyName>
    </creator>
  </creators>
  <titles>
    <title>Bovine Herpesvirus Type 4 (Bohv-4) Vector Delivering Nucleocapsid Protein Of Crimean-Congo Hemorrhagic Fever Virus Induces Comparable Protective Immunity Against Lethal Challenge In Ifn Alpha/Beta/Gamma R-/- Mice Models</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/71641</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3390/v11030237</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Crimean-Congo hemorrhagic fever virus (CCHFV) is the causative agent of a tick-borne infection with a significant mortality rate of up to 40% in endemic areas, with evidence of geographical expansion. Due to a lack of effective therapeutics and control measures, the development of a protective CCHFV vaccine remains a crucial public health task. This paper describes, for the first time, a Bovine herpesvirus type 4 (BoHV-4)-based viral vector (BoHV4- increment TK-CCHFV-N) and its immunogenicity in BALB/c and protection potential in IFN alpha/beta/gamma R-/- mice models in comparison with two routinely used vaccine platforms, namely, Adenovirus type 5 and a DNA vector (pCDNA3.1 myc/His A), expressing the same antigen. All vaccine constructs successfully elicited significantly elevated cytokine levels and specific antibody responses in immunized BALB/c and IFN alpha/beta/gamma R-/- mice. However, despite highly specific antibody responses in both animal models, the antibodies produced were unable to neutralize the virus in vitro. In the challenge experiment, only the BoHV4- increment TK-CCHFV-N and Ad5-N constructs produced 100% protection against lethal doses of the CCHFV Ank-2 strain in IFN alpha/beta/gamma R-/- mice. The delivery platforms could not be compared due to similar protection rates in IFN alpha/beta/gamma R-/- mice. However, during the challenge experiment in the T cell and passive antibody transfer assay, BoHV4- increment TK-CCHFV-N was dominant, with a protection rate of 75% compared to others. In conclusion, vector-based CCHFV N protein expression constitutes an effective approach for vaccine development and BoHV-4 emerged as a strong alternative to previously used viral vectors.</description>
  </descriptions>
</resource>