1 Ocak 2019 Dergi makalesi Açık Erişim

Farzani, Touraj Aligholipour; Foldes, Katalin; Hanifehnezhad, Alireza; Ilce, Burcu Yener; Dagalp, Seval Bilge; Khiabani, Neda Amirzadeh; Erguenay, Koray; Alkan, Feray; Karaoglu, Taner; Bodur, Hurrem; Ozkul, Aykut

Citation Style Language JSON

{
  "DOI": "10.3390/v11030237", 
  "abstract": "Crimean-Congo hemorrhagic fever virus (CCHFV) is the causative agent of a tick-borne infection with a significant mortality rate of up to 40% in endemic areas, with evidence of geographical expansion. Due to a lack of effective therapeutics and control measures, the development of a protective CCHFV vaccine remains a crucial public health task. This paper describes, for the first time, a Bovine herpesvirus type 4 (BoHV-4)-based viral vector (BoHV4- increment TK-CCHFV-N) and its immunogenicity in BALB/c and protection potential in IFN alpha/beta/gamma R-/- mice models in comparison with two routinely used vaccine platforms, namely, Adenovirus type 5 and a DNA vector (pCDNA3.1 myc/His A), expressing the same antigen. All vaccine constructs successfully elicited significantly elevated cytokine levels and specific antibody responses in immunized BALB/c and IFN alpha/beta/gamma R-/- mice. However, despite highly specific antibody responses in both animal models, the antibodies produced were unable to neutralize the virus in vitro. In the challenge experiment, only the BoHV4- increment TK-CCHFV-N and Ad5-N constructs produced 100% protection against lethal doses of the CCHFV Ank-2 strain in IFN alpha/beta/gamma R-/- mice. The delivery platforms could not be compared due to similar protection rates in IFN alpha/beta/gamma R-/- mice. However, during the challenge experiment in the T cell and passive antibody transfer assay, BoHV4- increment TK-CCHFV-N was dominant, with a protection rate of 75% compared to others. In conclusion, vector-based CCHFV N protein expression constitutes an effective approach for vaccine development and BoHV-4 emerged as a strong alternative to previously used viral vectors.", 
  "author": [
    {
      "family": "Farzani", 
      "given": " Touraj Aligholipour"
    }, 
    {
      "family": "Foldes", 
      "given": " Katalin"
    }, 
    {
      "family": "Hanifehnezhad", 
      "given": " Alireza"
    }, 
    {
      "family": "Ilce", 
      "given": " Burcu Yener"
    }, 
    {
      "family": "Dagalp", 
      "given": " Seval Bilge"
    }, 
    {
      "family": "Khiabani", 
      "given": " Neda Amirzadeh"
    }, 
    {
      "family": "Erguenay", 
      "given": " Koray"
    }, 
    {
      "family": "Alkan", 
      "given": " Feray"
    }, 
    {
      "family": "Karaoglu", 
      "given": " Taner"
    }, 
    {
      "family": "Bodur", 
      "given": " Hurrem"
    }, 
    {
      "family": "Ozkul", 
      "given": " Aykut"
    }
  ], 
  "container_title": "VIRUSES-BASEL", 
  "id": "71641", 
  "issue": "3", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "title": "Bovine Herpesvirus Type 4 (BoHV-4) Vector Delivering Nucleocapsid Protein of Crimean-Congo Hemorrhagic Fever Virus Induces Comparable Protective Immunity against Lethal Challenge in IFN alpha/beta/gamma R-/- Mice Models", 
  "type": "article-journal", 
  "volume": "11"
}