1 Ocak 2019 Dergi makalesi Açık Erişim

Ilem-Ozdemir, Derya; Atlihan-Gundogdu, Evren; Ekinci, Meliha; Halay, Erkan; Ay, Kadir; Karayildirim, Tamer; Asikoglu, Makbule

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{
  "@context": "https://schema.org/", 
  "@id": 67797, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Fac Pharm, Dept Radiopharm, Bornova, Turkey", 
      "name": "Ilem-Ozdemir, Derya"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Fac Pharm, Dept Radiopharm, Bornova, Turkey", 
      "name": "Atlihan-Gundogdu, Evren"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Fac Pharm, Dept Radiopharm, Bornova, Turkey", 
      "name": "Ekinci, Meliha"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Usak Univ, Sci Anal & Technol Applicat & Res Ctr, Usak, Turkey", 
      "name": "Halay, Erkan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Manisa Celal Bayar Univ, Fac Art & Sci, Dept Chem, Manisa, Turkey", 
      "name": "Ay, Kadir"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Fac Sci, Dept Chem, Bornova, Turkey", 
      "name": "Karayildirim, Tamer"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Fac Pharm, Dept Radiopharm, Bornova, Turkey", 
      "name": "Asikoglu, Makbule"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "The clinical impact and accessibility of Tc-99m tracers for cancer diagnosis would be greatly enhanced by the availability of a new, simple, and easy labeling process and radiopharmaceuticals. 5-Fluorouracil is an antitumor drug, which has played an important role for the treatment of breast carcinoma. In the present study, a new derivative of 5-Fluorouracil was synthesized as (1-[{1 '-(1 ''-deoxy-2 '',3 '':4 '',5 ''-di-O-isopropylidene-beta-D-fructopyranose-1 ''-yl)-1 ' H-1 ',2 ', 3 '-triazol-4 '-yl}methyl]-5-fluorouracil) (E) and radiolabeled with Tc-99m. It was analyzed by radio thin layer chromatography for quality control and stability. The radiolabeled complex was subjected to in vitro cell-binding studies to determine healthy and cancer cell affinity using HaCaT and MCF-7 cells, respectively. In addition, in vitro cytotoxicity studies of compound E were performed with HaCaT and MCF-5 cells. The radiochemical purity of the [Tc-99m]TcE was found to be higher than 90% at room temperature up to 6 hours. The radiolabeled complex showed higher specific binding to MCF-7 cells than HaCaT cells. IC50 values of E were found 31.5 +/- 3.4 mu M and 20.7 +/- 2.77 mu M for MCF-7 and HaCaT cells, respectively. The results demonstrated the potential of a new radiolabeled E with Tc-99m has selective for breast cancer cells.", 
  "headline": "Radiolabeling and in vitro evaluation of a new 5-fluorouracil derivative with cell culture studies", 
  "identifier": 67797, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Radiolabeling and in vitro evaluation of a new 5-fluorouracil derivative with cell culture studies", 
  "url": "https://aperta.ulakbim.gov.tr/record/67797"
}