1 Ocak 2019 Dergi makalesi Açık Erişim

Ilem-Ozdemir, Derya; Atlihan-Gundogdu, Evren; Ekinci, Meliha; Halay, Erkan; Ay, Kadir; Karayildirim, Tamer; Asikoglu, Makbule

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{
  "DOI": "10.1002/jlcr.3804", 
  "abstract": "The clinical impact and accessibility of Tc-99m tracers for cancer diagnosis would be greatly enhanced by the availability of a new, simple, and easy labeling process and radiopharmaceuticals. 5-Fluorouracil is an antitumor drug, which has played an important role for the treatment of breast carcinoma. In the present study, a new derivative of 5-Fluorouracil was synthesized as (1-[{1 '-(1 ''-deoxy-2 '',3 '':4 '',5 ''-di-O-isopropylidene-beta-D-fructopyranose-1 ''-yl)-1 ' H-1 ',2 ', 3 '-triazol-4 '-yl}methyl]-5-fluorouracil) (E) and radiolabeled with Tc-99m. It was analyzed by radio thin layer chromatography for quality control and stability. The radiolabeled complex was subjected to in vitro cell-binding studies to determine healthy and cancer cell affinity using HaCaT and MCF-7 cells, respectively. In addition, in vitro cytotoxicity studies of compound E were performed with HaCaT and MCF-5 cells. The radiochemical purity of the [Tc-99m]TcE was found to be higher than 90% at room temperature up to 6 hours. The radiolabeled complex showed higher specific binding to MCF-7 cells than HaCaT cells. IC50 values of E were found 31.5 +/- 3.4 mu M and 20.7 +/- 2.77 mu M for MCF-7 and HaCaT cells, respectively. The results demonstrated the potential of a new radiolabeled E with Tc-99m has selective for breast cancer cells.", 
  "author": [
    {
      "family": "Ilem-Ozdemir", 
      "given": " Derya"
    }, 
    {
      "family": "Atlihan-Gundogdu", 
      "given": " Evren"
    }, 
    {
      "family": "Ekinci", 
      "given": " Meliha"
    }, 
    {
      "family": "Halay", 
      "given": " Erkan"
    }, 
    {
      "family": "Ay", 
      "given": " Kadir"
    }, 
    {
      "family": "Karayildirim", 
      "given": " Tamer"
    }, 
    {
      "family": "Asikoglu", 
      "given": " Makbule"
    }
  ], 
  "container_title": "JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS", 
  "id": "67797", 
  "issue": "13", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "page": "874-884", 
  "title": "Radiolabeling and in vitro evaluation of a new 5-fluorouracil derivative with cell culture studies", 
  "type": "article-journal", 
  "volume": "62"
}