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Phthalocyanine-based mesoporous organosilica nanoparticles: NIR photodynamic efficiency and siRNA photochemical internalization

2019    Ekineker, Guelcin; Nguyen, Christophe; Bayir, Suemeyra; Dominguez Gil, Sofia; Isci, Umit; Daurat, Morgane; Godefroy, Anastasia; Raehm, Laurence; Charnay, Clarence; Oliviero, Erwan; Ahsen, Vefa; Gary-Bobo, Magali; Durand, Jean-Olivier; Dumoulin, Fabienne

Mesoporous organosilica nanoparticles (PHT-PMO) have been prepared from an octa-triethoxysilylated Zn phthalocyanine precursor. These PHT-PMO nanoparticles had no dark toxicity but high phototoxicity when irradiated at 650 nm, and remarkable near-infrared phototoxicity when excited at 760 and 810 nm. The PHT-PMO were then aminated to promote electrostatic complexation with siRNA. Transfection experiments were performed upon NIR irradiation and photochemical internalization was very efficient, leading to 65% luciferase extinction in MCF-7 cancer cells expressing stable luciferase.