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Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

Dervisevic, Muamer; Cevik, Emre; Senel, Mehmet; Nergiz, Cevdet; Abasiyanik, M. Fatih


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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Dervisevic, Muamer</dc:creator>
  <dc:creator>Cevik, Emre</dc:creator>
  <dc:creator>Senel, Mehmet</dc:creator>
  <dc:creator>Nergiz, Cevdet</dc:creator>
  <dc:creator>Abasiyanik, M. Fatih</dc:creator>
  <dc:date>2016-01-01</dc:date>
  <dc:description>An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinudeotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiephene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic add. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed. (C) 2016 Elsevier B.V. All rights reserved.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/56497</dc:identifier>
  <dc:identifier>oai:zenodo.org:56497</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF ELECTROANALYTICAL CHEMISTRY 776 18-24</dc:source>
  <dc:title>Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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