Dergi makalesi Açık Erişim
Dervisevic, Muamer; Cevik, Emre; Senel, Mehmet; Nergiz, Cevdet; Abasiyanik, M. Fatih
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<identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/56497</identifier>
<creators>
<creator>
<creatorName>Dervisevic, Muamer</creatorName>
<givenName>Muamer</givenName>
<familyName>Dervisevic</familyName>
<affiliation>Fatih Univ, Inst Biomed Engn, TR-34500 Istanbul, Turkey</affiliation>
</creator>
<creator>
<creatorName>Cevik, Emre</creatorName>
<givenName>Emre</givenName>
<familyName>Cevik</familyName>
<affiliation>Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey</affiliation>
</creator>
<creator>
<creatorName>Senel, Mehmet</creatorName>
<givenName>Mehmet</givenName>
<familyName>Senel</familyName>
<affiliation>Fatih Univ, Inst Biomed Engn, TR-34500 Istanbul, Turkey</affiliation>
</creator>
<creator>
<creatorName>Nergiz, Cevdet</creatorName>
<givenName>Cevdet</givenName>
<familyName>Nergiz</familyName>
<affiliation>Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey</affiliation>
</creator>
<creator>
<creatorName>Abasiyanik, M. Fatih</creatorName>
<givenName>M. Fatih</givenName>
<familyName>Abasiyanik</familyName>
<affiliation>Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey</affiliation>
</creator>
</creators>
<titles>
<title>Amperometric Cholesterol Biosensor Based On Reconstituted Cholesterol Oxidase On Boronic Acid Functional Conducting Polymers</title>
</titles>
<publisher>Aperta</publisher>
<publicationYear>2016</publicationYear>
<dates>
<date dateType="Issued">2016-01-01</date>
</dates>
<resourceType resourceTypeGeneral="Text">Journal article</resourceType>
<alternateIdentifiers>
<alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/56497</alternateIdentifier>
</alternateIdentifiers>
<relatedIdentifiers>
<relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.jelechem.2016.06.033</relatedIdentifier>
</relatedIdentifiers>
<rightsList>
<rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
<rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
</rightsList>
<descriptions>
<description descriptionType="Abstract">An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinudeotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiephene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic add. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed. (C) 2016 Elsevier B.V. All rights reserved.</description>
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