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Biocontrol of Shiga Toxigenic Escherichia coli O157:H7 in Turkish Raw Meatball by Bacteriophage

Gencay, Yilmaz Emre; Ayaz, Naim Deniz; Copuroglu, Gizem; Erol, Irfan


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/54033</identifier>
  <creators>
    <creator>
      <creatorName>Gencay, Yilmaz Emre</creatorName>
      <givenName>Yilmaz Emre</givenName>
      <familyName>Gencay</familyName>
      <affiliation>Kirikkale Univ, Fac Vet Med, Dept Food Hyg &amp; Technol, TR-71450 Yahsihan, Kirikkale, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ayaz, Naim Deniz</creatorName>
      <givenName>Naim Deniz</givenName>
      <familyName>Ayaz</familyName>
      <affiliation>Kirikkale Univ, Fac Vet Med, Dept Food Hyg &amp; Technol, TR-71450 Yahsihan, Kirikkale, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Copuroglu, Gizem</creatorName>
      <givenName>Gizem</givenName>
      <familyName>Copuroglu</familyName>
      <affiliation>Kirikkale Univ, Fac Vet Med, Dept Food Hyg &amp; Technol, TR-71450 Yahsihan, Kirikkale, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erol, Irfan</creatorName>
      <givenName>Irfan</givenName>
      <familyName>Erol</familyName>
      <affiliation>Republ Turkey Minist Food Agr &amp; Livestock, Gen Directorate Food &amp; Control, Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Biocontrol Of Shiga Toxigenic Escherichia Coli O157:H7 In Turkish Raw Meatball By Bacteriophage</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2016</publicationYear>
  <dates>
    <date dateType="Issued">2016-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/54033</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1111/jfs.12219</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">With an Escherichia coli O157:H7 virulent bacteriophage, M8AEC16, biocontrol efficiency of phages on a highly risky, ready-to-eat, traditional delicacy food called raw meatball under different storage conditions was investigated. Phage, belonging to the Myoviridae family, was isolated from the wastewater of a local slaughterhouse and showed a broad lytic activity toward many E.coli O157:H7 strains with high efficiency of plating and O157 specificity. Our experimental study provided favorable results, with 0.69-2.09 log colony-forming unit (cfu)/g E.coli O157:H7 reductions in the first 5h of the replica trials. Major reductions of viable E.coli O157:H7 counts were observed in the beginning of the storage period, reaching up to 1.85 log cfu/g. Although a significant reduction in E.coli O157:H7 was observed with increased phage concentration, storage conditions had minor effect on efficiency of phage biocontrol. This is the first study in Turkey that investigates applicability of phage biocontrol for a traditional food model.</description>
  </descriptions>
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