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Biocontrol of Shiga Toxigenic Escherichia coli O157:H7 in Turkish Raw Meatball by Bacteriophage

Gencay, Yilmaz Emre; Ayaz, Naim Deniz; Copuroglu, Gizem; Erol, Irfan


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{
  "DOI": "10.1111/jfs.12219", 
  "abstract": "With an Escherichia coli O157:H7 virulent bacteriophage, M8AEC16, biocontrol efficiency of phages on a highly risky, ready-to-eat, traditional delicacy food called raw meatball under different storage conditions was investigated. Phage, belonging to the Myoviridae family, was isolated from the wastewater of a local slaughterhouse and showed a broad lytic activity toward many E.coli O157:H7 strains with high efficiency of plating and O157 specificity. Our experimental study provided favorable results, with 0.69-2.09 log colony-forming unit (cfu)/g E.coli O157:H7 reductions in the first 5h of the replica trials. Major reductions of viable E.coli O157:H7 counts were observed in the beginning of the storage period, reaching up to 1.85 log cfu/g. Although a significant reduction in E.coli O157:H7 was observed with increased phage concentration, storage conditions had minor effect on efficiency of phage biocontrol. This is the first study in Turkey that investigates applicability of phage biocontrol for a traditional food model.", 
  "author": [
    {
      "family": "Gencay", 
      "given": " Yilmaz Emre"
    }, 
    {
      "family": "Ayaz", 
      "given": " Naim Deniz"
    }, 
    {
      "family": "Copuroglu", 
      "given": " Gizem"
    }, 
    {
      "family": "Erol", 
      "given": " Irfan"
    }
  ], 
  "container_title": "JOURNAL OF FOOD SAFETY", 
  "id": "54033", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2016, 
        1, 
        1
      ]
    ]
  }, 
  "page": "120-131", 
  "title": "Biocontrol of Shiga Toxigenic Escherichia coli O157:H7 in Turkish Raw Meatball by Bacteriophage", 
  "type": "article-journal", 
  "volume": "36"
}
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