1 Ocak 2017 Dergi makalesi Açık Erişim

Yaman, Sena; Calik, Pinar

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Yaman, Sena</dc:creator>
  <dc:creator>Calik, Pinar</dc:creator>
  <dc:date>2017-01-01</dc:date>
  <dc:description>The aim of this work was to develop an effective fed-batch feeding strategy to enhance recombinant glucose isomerase (r-GI) production by recombinant Escherichia coli BL21 (DE3) pLysS on an industrially relevant feedstock without the application of an exogenous inducer. Following the batch operation (0 &lt; t &lt; 7H), the effects of pulse and/or continuous feeding of hydrolyzed beet molasses were investigated under five different feeding strategies. The two most promising strategies with respect to r-GI activity were (i) PM-0.05, designed with one pulse feed (t = 7H) followed by a continuous feed and (ii) 2PM(F)-0.05, designed with two consecutive pulse feeds (t = 7 and 10H) followed by a continuous feed. The continuous feeding of molasses for both fermentation strategies employed the same precalculated feeding rate, (o) = 0.05H(-1). The maximum r-GI activities exhibited by PM-0.05 and 2PM(F)-0.05 were 29,050 and 30,642U dm(-3), respectively. On the one hand, compared to PM-0.05 r-GI activity reached its maximum within a shorter cultivation time (t(max) = 2H) at 2PM(F)-0.05, which could be preferable in terms of manufacturing costs and possible risks; on the other hand, PM-0.05 is a simpler fermentation regime compared to 2PM(F)-0.05 with respect to manipulations that should be considered in large-scale production. (C) 2017 International Union of Biochemistry and Molecular Biology, Inc.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/49611</dc:identifier>
  <dc:identifier>oai:zenodo.org:49611</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 64(6) 944-954</dc:source>
  <dc:title>Beet molasses-based feeding strategy enhances recombinant thermostable glucose isomerase production by Escherichia coli BL21 (DE3)</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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