1 Ocak 2017 Dergi makalesi Açık Erişim

Yaman, Sena; Calik, Pinar

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/49611</identifier>
  <creators>
    <creator>
      <creatorName>Yaman, Sena</creatorName>
      <givenName>Sena</givenName>
      <familyName>Yaman</familyName>
    </creator>
    <creator>
      <creatorName>Calik, Pinar</creatorName>
      <givenName>Pinar</givenName>
      <familyName>Calik</familyName>
    </creator>
  </creators>
  <titles>
    <title>Beet Molasses-Based Feeding Strategy Enhances Recombinant Thermostable Glucose Isomerase Production By Escherichia Coli Bl21 (De3)</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/49611</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1002/bab.1549</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">The aim of this work was to develop an effective fed-batch feeding strategy to enhance recombinant glucose isomerase (r-GI) production by recombinant Escherichia coli BL21 (DE3) pLysS on an industrially relevant feedstock without the application of an exogenous inducer. Following the batch operation (0 &amp;lt; t &amp;lt; 7H), the effects of pulse and/or continuous feeding of hydrolyzed beet molasses were investigated under five different feeding strategies. The two most promising strategies with respect to r-GI activity were (i) PM-0.05, designed with one pulse feed (t = 7H) followed by a continuous feed and (ii) 2PM(F)-0.05, designed with two consecutive pulse feeds (t = 7 and 10H) followed by a continuous feed. The continuous feeding of molasses for both fermentation strategies employed the same precalculated feeding rate, (o) = 0.05H(-1). The maximum r-GI activities exhibited by PM-0.05 and 2PM(F)-0.05 were 29,050 and 30,642U dm(-3), respectively. On the one hand, compared to PM-0.05 r-GI activity reached its maximum within a shorter cultivation time (t(max) = 2H) at 2PM(F)-0.05, which could be preferable in terms of manufacturing costs and possible risks; on the other hand, PM-0.05 is a simpler fermentation regime compared to 2PM(F)-0.05 with respect to manipulations that should be considered in large-scale production. (C) 2017 International Union of Biochemistry and Molecular Biology, Inc.</description>
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