1 Ocak 2017 Dergi makalesi Açık Erişim

Aktas, Erdem; Chamberlain, Connie S.; Saether, Erin E.; Duenwald-Kuehl, Sarah E.; Kondratko-Mittnacht, Jaclyn; Stitgen, Michael; Lee, Jae Sung; Clements, Anna E.; Murphy, William L.; Vanderby, Ray

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/46743</identifier>
  <creators>
    <creator>
      <creatorName>Aktas, Erdem</creatorName>
      <givenName>Erdem</givenName>
      <familyName>Aktas</familyName>
      <affiliation>Ankara Oncol Res &amp; Training Hosp, Dept Orthoped, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Chamberlain, Connie S.</creatorName>
      <givenName>Connie S.</givenName>
      <familyName>Chamberlain</familyName>
      <affiliation>Univ Wisconsin, Dept Orthoped &amp; Rehabil, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Saether, Erin E.</creatorName>
      <givenName>Erin E.</givenName>
      <familyName>Saether</familyName>
      <affiliation>Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Duenwald-Kuehl, Sarah E.</creatorName>
      <givenName>Sarah E.</givenName>
      <familyName>Duenwald-Kuehl</familyName>
      <affiliation>Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Kondratko-Mittnacht, Jaclyn</creatorName>
      <givenName>Jaclyn</givenName>
      <familyName>Kondratko-Mittnacht</familyName>
      <affiliation>Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Stitgen, Michael</creatorName>
      <givenName>Michael</givenName>
      <familyName>Stitgen</familyName>
      <affiliation>Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Lee, Jae Sung</creatorName>
      <givenName>Jae Sung</givenName>
      <familyName>Lee</familyName>
      <affiliation>Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Clements, Anna E.</creatorName>
      <givenName>Anna E.</givenName>
      <familyName>Clements</familyName>
      <affiliation>Univ Wisconsin, Dept Orthoped &amp; Rehabil, Madison, WI 53705 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Murphy, William L.</creatorName>
      <givenName>William L.</givenName>
      <familyName>Murphy</familyName>
    </creator>
    <creator>
      <creatorName>Vanderby, Ray</creatorName>
      <givenName>Ray</givenName>
      <familyName>Vanderby</familyName>
    </creator>
  </creators>
  <titles>
    <title>Immune Modulation With Primed Mesenchymal Stem Cells Delivered Via Biodegradable Scaffold To Repair An Achilles Tendon Segmental Defect</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2017</publicationYear>
  <dates>
    <date dateType="Issued">2017-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/46743</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1002/jor.23258</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Tendon healing is a complex coordinated series of events resulting in protracted recovery, limited regeneration, and scar formation. Mesenchymal stem cell (MSC) therapy has shown promise as a new technology to enhance soft tissue and bone healing. A challenge with MSC therapy involves the ability to consistently control the inflammatory response and subsequent healing. Previous studies suggest that preconditioning MSCs with inflammatory cytokines, such as IFN-, TNF-, and IL-1 may accelerate cutaneous wound closure. The objective of this study was to therefore elucidate these effects in tendon. That is, the in vivo healing effects of TNF- primed MSCs were studied using a rat Achilles segmental defect model. Rat Achilles tendons were subjected to a unilateral 3mm segmental defect and repaired with either a PLG scaffold alone, MSC-seeded PLG scaffold, or TNF--primed MSC-seeded PLG scaffold. Achilles tendons were analyzed at 2 and 4 weeks post-injury. In vivo, MSCs, regardless of priming, increased IL-10 production and reduced the inflammatory factor, IL-1. Primed MSCs reduced IL-12 production and the number of M1 macrophages, as well as increased the percent of M2 macrophages, and synthesis of the anti-inflammatory factor IL-4. Primed MSC treatment also increased the concentration of type I procollagen in the healing tissue and increased failure stress of the tendon 4 weeks post-injury. Taken together delivery of TNF- primed MSCs via 3D PLG scaffold modulated macrophage polarization and cytokine production to further accentuate the more regenerative MSC-induced healing response. (c) 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:269-280, 2017.</description>
  </descriptions>
</resource>