1 Ocak 2018 Dergi makalesi Açık Erişim

Eren, Erden; Tufekci, Kemal Ugur; Isci, Kamer Burak; Tastan, Bora; Genc, Kursad; Genc, Sermin

JSON

{
  "conceptrecid": "36752", 
  "created": "2021-03-15T19:39:56.096246+00:00", 
  "doi": "10.3389/fimmu.2018.00036", 
  "files": [
    {
      "bucket": "1ae153c5-207c-4af0-aff5-faf1f05d891c", 
      "checksum": "md5:c92854c914b13c8a6bf6345246288ad5", 
      "key": "bib-c52a2ccc-d0b4-429f-a2d3-702ec3e523d3.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/1ae153c5-207c-4af0-aff5-faf1f05d891c/bib-c52a2ccc-d0b4-429f-a2d3-702ec3e523d3.txt"
      }, 
      "size": 424, 
      "type": "txt"
    }
  ], 
  "id": 36753, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.3389/fimmu.2018.00036.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/1ae153c5-207c-4af0-aff5-faf1f05d891c", 
    "doi": "https://doi.org/10.3389/fimmu.2018.00036", 
    "html": "https://aperta.ulakbim.gov.tr/record/36753", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/36753", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/36753"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-destekli-proje-yayinlari"
      }
    ], 
    "creators": [
      {
        "name": "Eren, Erden"
      }, 
      {
        "name": "Tufekci, Kemal Ugur"
      }, 
      {
        "name": "Isci, Kamer Burak"
      }, 
      {
        "name": "Tastan, Bora"
      }, 
      {
        "affiliation": "Dokuz Eylul Univ, Hlth Sci Inst, Dept Neurosci, Izmir, Turkey", 
        "name": "Genc, Kursad"
      }, 
      {
        "name": "Genc, Sermin"
      }
    ], 
    "description": "Sulforaphane (SFN) is a natural product with cytoprotective, anti-inflammatory, and antioxidant effects. In this study, we evaluated the mechanisms of its effects on lipopolysaccharide (LPS)-induced cell death, inflammation, oxidative stress, and polarization in murine microglia. We found that SFN protects N9 microglial cells upon LPS-induced cell death and suppresses LPS-induced levels of secreted pro-inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. SFN is also a potent inducer of redox sensitive transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), which is responsible for the transcription of antioxidant, cytoprotective, and anti-inflammatory genes. SFN induced translocation of Nrf2 to the nucleus via extracellular signal-regulated kinase 1/2 (ERK1/2) pathway activation. siRNA-mediated knockdown study showed that the effects of SFN on LPS-induced reactive oxygen species, reactive nitrogen species, and pro-inflammatory cytokine production and cell death are partly Nrf2 dependent. Mox phenotype is a novel microglial phenotype that has roles in oxidative stress responses. Our results suggested that SFN induced the Mox phenotype in murine microglia through Nrf2 pathway. SFN also alleviated LPS-induced expression of inflammatory microRNA, miR-155. Finally, SFN inhibits microglia-mediated neurotoxicity as demonstrated by conditioned medium and co-culture experiments. In conclusion, SFN exerts protective effects on microglia and modulates the microglial activation state.", 
    "doi": "10.3389/fimmu.2018.00036", 
    "has_grant": false, 
    "journal": {
      "title": "FRONTIERS IN IMMUNOLOGY", 
      "volume": "9"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2018-01-01", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "36753"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "36752"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "Sulforaphane Inhibits Lipopolysaccharide-Induced Inflammation, Cytotoxicity, Oxidative Stress, and miR-155 Expression and Switches to Mox Phenotype through Activating Extracellular Signal-Regulated Kinase 1/2-Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway in Murine Microglial Cells"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 42.0, 
    "unique_downloads": 42.0, 
    "unique_views": 179.0, 
    "version_downloads": 42.0, 
    "version_unique_downloads": 42.0, 
    "version_unique_views": 179.0, 
    "version_views": 230.0, 
    "version_volume": 17808.0, 
    "views": 230.0, 
    "volume": 17808.0
  }, 
  "updated": "2021-03-15T19:39:56.136033+00:00"
}