Dergi makalesi Açık Erişim
Eren, Erden; Tufekci, Kemal Ugur; Isci, Kamer Burak; Tastan, Bora; Genc, Kursad; Genc, Sermin
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/36753</identifier> <creators> <creator> <creatorName>Eren, Erden</creatorName> <givenName>Erden</givenName> <familyName>Eren</familyName> </creator> <creator> <creatorName>Tufekci, Kemal Ugur</creatorName> <givenName>Kemal Ugur</givenName> <familyName>Tufekci</familyName> </creator> <creator> <creatorName>Isci, Kamer Burak</creatorName> <givenName>Kamer Burak</givenName> <familyName>Isci</familyName> </creator> <creator> <creatorName>Tastan, Bora</creatorName> <givenName>Bora</givenName> <familyName>Tastan</familyName> </creator> <creator> <creatorName>Genc, Kursad</creatorName> <givenName>Kursad</givenName> <familyName>Genc</familyName> <affiliation>Dokuz Eylul Univ, Hlth Sci Inst, Dept Neurosci, Izmir, Turkey</affiliation> </creator> <creator> <creatorName>Genc, Sermin</creatorName> <givenName>Sermin</givenName> <familyName>Genc</familyName> </creator> </creators> <titles> <title>Sulforaphane Inhibits Lipopolysaccharide-Induced Inflammation, Cytotoxicity, Oxidative Stress, And Mir-155 Expression And Switches To Mox Phenotype Through Activating Extracellular Signal-Regulated Kinase 1/2-Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway In Murine Microglial Cells</title> </titles> <publisher>Aperta</publisher> <publicationYear>2018</publicationYear> <dates> <date dateType="Issued">2018-01-01</date> </dates> <resourceType resourceTypeGeneral="Text">Journal article</resourceType> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/36753</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3389/fimmu.2018.00036</relatedIdentifier> </relatedIdentifiers> <rightsList> <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract">Sulforaphane (SFN) is a natural product with cytoprotective, anti-inflammatory, and antioxidant effects. In this study, we evaluated the mechanisms of its effects on lipopolysaccharide (LPS)-induced cell death, inflammation, oxidative stress, and polarization in murine microglia. We found that SFN protects N9 microglial cells upon LPS-induced cell death and suppresses LPS-induced levels of secreted pro-inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. SFN is also a potent inducer of redox sensitive transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), which is responsible for the transcription of antioxidant, cytoprotective, and anti-inflammatory genes. SFN induced translocation of Nrf2 to the nucleus via extracellular signal-regulated kinase 1/2 (ERK1/2) pathway activation. siRNA-mediated knockdown study showed that the effects of SFN on LPS-induced reactive oxygen species, reactive nitrogen species, and pro-inflammatory cytokine production and cell death are partly Nrf2 dependent. Mox phenotype is a novel microglial phenotype that has roles in oxidative stress responses. Our results suggested that SFN induced the Mox phenotype in murine microglia through Nrf2 pathway. SFN also alleviated LPS-induced expression of inflammatory microRNA, miR-155. Finally, SFN inhibits microglia-mediated neurotoxicity as demonstrated by conditioned medium and co-culture experiments. In conclusion, SFN exerts protective effects on microglia and modulates the microglial activation state.</description> </descriptions> </resource>
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