1 Ocak 2018 Dergi makalesi Açık Erişim

Eminoglu, Aysenur; Akturk Dizman, Yesim; Guzel, Sule; Belduz, Ali Osman

Citation Style Language JSON

{
  "DOI": "10.3906/biy-1606-54", 
  "abstract": "Cytochrome P450 monooxygenases are one of the largest heme-containing protein groups, and the majority of them catalyze hydroxylation reactions dependent on nicotinamide adenine dinucleotide phosphate and oxygen. Cytochrome P450 (CYP) enzymes function in a wide range of monooxygenation reactions essential in primary and secondary metabolism in plants. Camellia sinensis (L.) Kuntze is a commercially and economically valuable plant due to its medicinally important secondary metabolites and as a beloved beverage. Cytochrome P450 monooxygenases play a significant role in the biosynthesis of a variety of secondary metabolites in tea. Although the biosynthesis of secondary metabolites has been investigated in detail, there have been limited studies conducted on identifying the genetic mechanisms of CYP-catalyzed secondary metabolic pathways in the C. sinensis (tea) plant. In our study, we characterized a putative C. sinensis (L.) Kuntze cytochrome P450 monooxygenase gene (Csp450), which has 1759 bp full-length cDNA with 49 bp of 5 ' and 183 bp of 3 ' untranslated regions. The CDS of the gene is 1527 bp and 508 amino acids in length. BLAST results of the deduced amino acid sequence revealed a high similarity with the CYP704C1-like superfamily. Preharvest period gene expression analysis from May, July, and September did not show any difference.", 
  "author": [
    {
      "family": "Eminoglu", 
      "given": " Aysenur"
    }, 
    {
      "family": "Akturk Dizman", 
      "given": " Yesim"
    }, 
    {
      "family": "Guzel", 
      "given": " Sule"
    }, 
    {
      "family": "Belduz", 
      "given": " Ali Osman"
    }
  ], 
  "container_title": "TURKISH JOURNAL OF BIOLOGY", 
  "id": "30267", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "page": "1-11", 
  "title": "Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)", 
  "type": "article-journal", 
  "volume": "42"
}