1 Ocak 2023 Dergi makalesi Açık Erişim

Onbas, Rabia; Arslan Yildiz, Ahu

DataCite XML

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/269022</identifier>
  <creators>
    <creator>
      <creatorName>Onbas, Rabia</creatorName>
      <givenName>Rabia</givenName>
      <familyName>Onbas</familyName>
      <affiliation>Izmir Inst Technol IZTECH, Dept Bioengn, TR-35430 Izmir, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Arslan Yildiz, Ahu</creatorName>
      <givenName>Ahu</givenName>
      <familyName>Arslan Yildiz</familyName>
      <affiliation>Izmir Inst Technol IZTECH, Dept Bioengn, TR-35430 Izmir, Turkiye</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Biopatterning Of 3D Cellular Model By Contactless Magnetic Manipulation For Cardiotoxicity Screening</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2023</publicationYear>
  <dates>
    <date dateType="Issued">2023-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/269022</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1089/ten.tea.2023.0197</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Patterning cells to create three-dimensional (3D) cell culture models by magnetic manipulation is a promising technique, which is rapid, simple, and cost-effective. This study introduces a new biopatterning approach based on magnetic manipulation of cells with a bioink that consists alginate, cells, and magnetic nanoparticles. Plackett-Burman and Box-Behnken experimental design models were used to optimize bioink formulation where NIH-3T3 cells were utilized as a model cell line. The patterning capability was confirmed by light microscopy through 7 days culture time. Then, biopatterned 3D cardiac structures were formed using H9c2 cardiomyocyte cells. Cellular and extracellular components, F-actin and collagen Type I, and cardiac-specific biomarkers, Troponin T and MYH6, of biopatterned 3D cardiac structures were observed successfully. Moreover, Doxorubicin (DOX)-induced cardiotoxicity was investigated for developed 3D model, and IC50 value was calculated as 8.1 mu M for biopatterned 3D cardiac structures, which showed higher resistance against DOX-exposure compared to conventional two-dimensional cell culture. Hereby, developed biopatterning methodology proved to be a simple and rapid approach to fabricate 3D cardiac models, especially for drug screening applications.&lt;/p&gt;
&lt;p&gt;Impact statementContactless manipulation and cell patterning techniques provide rapid and cost-effective three-dimensional (3D) cell culture model formation for tissue engineering applications. The present study introduces a new methodology that comprised alginate-based bioink to pattern cells via contactless magnetic manipulation to fabricate 3D cardiac structures. The developed cardiac model was evaluated in terms of Doxorubicin-induced cardiotoxicity and biopatterned 3D cardiac structures were found more resistant to drug exposure compared to two-dimensional control.&lt;/p&gt;</description>
  </descriptions>
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