Dergi makalesi Açık Erişim
Onbas, Rabia; Arslan Yildiz, Ahu
{ "DOI": "10.1089/ten.tea.2023.0197", "abstract": "<p>Patterning cells to create three-dimensional (3D) cell culture models by magnetic manipulation is a promising technique, which is rapid, simple, and cost-effective. This study introduces a new biopatterning approach based on magnetic manipulation of cells with a bioink that consists alginate, cells, and magnetic nanoparticles. Plackett-Burman and Box-Behnken experimental design models were used to optimize bioink formulation where NIH-3T3 cells were utilized as a model cell line. The patterning capability was confirmed by light microscopy through 7 days culture time. Then, biopatterned 3D cardiac structures were formed using H9c2 cardiomyocyte cells. Cellular and extracellular components, F-actin and collagen Type I, and cardiac-specific biomarkers, Troponin T and MYH6, of biopatterned 3D cardiac structures were observed successfully. Moreover, Doxorubicin (DOX)-induced cardiotoxicity was investigated for developed 3D model, and IC50 value was calculated as 8.1 mu M for biopatterned 3D cardiac structures, which showed higher resistance against DOX-exposure compared to conventional two-dimensional cell culture. Hereby, developed biopatterning methodology proved to be a simple and rapid approach to fabricate 3D cardiac models, especially for drug screening applications.</p>\n<p>Impact statementContactless manipulation and cell patterning techniques provide rapid and cost-effective three-dimensional (3D) cell culture model formation for tissue engineering applications. The present study introduces a new methodology that comprised alginate-based bioink to pattern cells via contactless magnetic manipulation to fabricate 3D cardiac structures. The developed cardiac model was evaluated in terms of Doxorubicin-induced cardiotoxicity and biopatterned 3D cardiac structures were found more resistant to drug exposure compared to two-dimensional control.</p>", "author": [ { "family": "Onbas", "given": " Rabia" }, { "family": "Arslan Yildiz", "given": " Ahu" } ], "container_title": "TISSUE ENGINEERING PART A", "id": "269022", "issued": { "date-parts": [ [ 2023, 1, 1 ] ] }, "page": "10", "title": "Biopatterning of 3D Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening", "type": "article-journal" }
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