1 Ocak 2010 Dergi makalesi Açık Erişim

Gungormusler, Mine; Gonen, Cagdas; Ozdemir, Guven; Azbar, Nuri

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        "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, TR-35100 Izmir, Turkey", 
        "name": "Gungormusler, Mine"
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        "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, TR-35100 Izmir, Turkey", 
        "name": "Gonen, Cagdas"
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      {
        "affiliation": "Ege Univ, Dept Biol, Fac Sci, TR-35100 Izmir, Turkey", 
        "name": "Ozdemir, Guven"
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      {
        "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, TR-35100 Izmir, Turkey", 
        "name": "Azbar, Nuri"
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    "description": "Klebsiella species are excellent 1,3-propanediol (1,3 PDO) producers. Klebsiella pneumoniae is a well-studied microorganism and, therefore, its metabolic pathway is known. Accordingly, a number of by-products are simultaneously produced together with 1,3 PDO. Optimization of medium composition significantly decreased these by-products' concentrations. Optimization of culture media concentrations on 1,3 PDO production by K. pneumoniae was studied using statistical experimental designs and analysis, namely, Taguchi Orthogonal Array (TOA) and Box-Behnken design (BBD). The optimal composition of the fermentation medium for maximum 1,3 PDO yield, with a LIS (2 boolean AND 1 x 3 boolean AND 7) design using TOA, and after that, a three-level three-factor BBD, was obtained. The most significant chemicals were found to be glycerol (p<0.0001) and K2HPO4 (p<0.05). MgSO4 center dot 7H(2)0, which is the third significant factor obtained from TOA, was not found to be significant (p>0.05) from BBD. A maximum 1,3 PDO concentration of 23.6 g/L was obtained which is higher compared to that foreseen by the statistical model (9.3 g/L).", 
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      "issue": "12", 
      "pages": "2840-2847", 
      "title": "FRESENIUS ENVIRONMENTAL BULLETIN", 
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    "title": "FERMENTATION MEDIUM OPTIMIZATION FOR 1,3-PROPANEDIOL PRODUCTION USING TAGUCHI AND BOX-BEHNKEN EXPERIMENTAL DESIGNS"
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