1 Ocak 2022 Dergi makalesi Açık Erişim

Zhou, Jing; Kong, Yong; Wu, Mengmeng; Shu, Fengyue; Wang, Haijun; Ma, Shuonan; Li, Yan; Jeppesen, Erik

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  <dc:creator>Zhou, Jing</dc:creator>
  <dc:creator>Kong, Yong</dc:creator>
  <dc:creator>Wu, Mengmeng</dc:creator>
  <dc:creator>Shu, Fengyue</dc:creator>
  <dc:creator>Wang, Haijun</dc:creator>
  <dc:creator>Ma, Shuonan</dc:creator>
  <dc:creator>Li, Yan</dc:creator>
  <dc:creator>Jeppesen, Erik</dc:creator>
  <dc:date>2022-01-01</dc:date>
  <dc:description>Excessive nitrogen (N) input is an important factor influencing aquatic ecosystems and has received increasing public attention in the past decades. It remains unclear how N input affects the denitrifying bacterial communities that play a key role in regulating N cycles in various ecosystems. To test our hypothesis-that the abundance and biodiversity of denitrifying bacterial communities decrease with increasing N-we compared the abundance and composition of denitrifying bacteria having nitrous oxide reductase gene (nosZ I) from sediments (0-20 cm) in five experimental ponds with different nitrogen fertilization treatment (TN10, TN20, TN30, TN40, TN50) using quantitative PCR and pyrosequencing techniques. We found that (1) N addition significantly decreased nosZ I gene abundance, (2) the Invsimpson and Shannon indices (reflecting biodiversity) first increased significantly along with the increasing N loading in TN10-TN40 followed by a decrease in TN50, (3) the beta diversity of the nosZ I denitrifier was clustered into three groups along the TN concentration levels: Cluster I (TN50), Cluster II (TN40), and Cluster III (TN10-TN30), (4) the proportions of Alphaproteobacteria and Betaproteobacteria in the high-N treatment (TN50) were significantly lower than in the lower N treatments (TN10-TN30). (5) The TN concentration was the most important factor driving the alteration of denitrifying bacteria assemblages. Our findings shed new light on the response of denitrification-related bacteria to long-term N loading at pond scale and on the response of denitrifying microorganisms to N pollution.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/258735</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:258735</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>MICROBIAL ECOLOGY</dc:source>
  <dc:title>Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): a Long-Term Pond Experiment</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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