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Zhou, Jing; Kong, Yong; Wu, Mengmeng; Shu, Fengyue; Wang, Haijun; Ma, Shuonan; Li, Yan; Jeppesen, Erik
{ "DOI": "10.1007/s00248-022-01971-4", "abstract": "Excessive nitrogen (N) input is an important factor influencing aquatic ecosystems and has received increasing public attention in the past decades. It remains unclear how N input affects the denitrifying bacterial communities that play a key role in regulating N cycles in various ecosystems. To test our hypothesis-that the abundance and biodiversity of denitrifying bacterial communities decrease with increasing N-we compared the abundance and composition of denitrifying bacteria having nitrous oxide reductase gene (nosZ I) from sediments (0-20 cm) in five experimental ponds with different nitrogen fertilization treatment (TN10, TN20, TN30, TN40, TN50) using quantitative PCR and pyrosequencing techniques. We found that (1) N addition significantly decreased nosZ I gene abundance, (2) the Invsimpson and Shannon indices (reflecting biodiversity) first increased significantly along with the increasing N loading in TN10-TN40 followed by a decrease in TN50, (3) the beta diversity of the nosZ I denitrifier was clustered into three groups along the TN concentration levels: Cluster I (TN50), Cluster II (TN40), and Cluster III (TN10-TN30), (4) the proportions of Alphaproteobacteria and Betaproteobacteria in the high-N treatment (TN50) were significantly lower than in the lower N treatments (TN10-TN30). (5) The TN concentration was the most important factor driving the alteration of denitrifying bacteria assemblages. Our findings shed new light on the response of denitrification-related bacteria to long-term N loading at pond scale and on the response of denitrifying microorganisms to N pollution.", "author": [ { "family": "Zhou", "given": " Jing" }, { "family": "Kong", "given": " Yong" }, { "family": "Wu", "given": " Mengmeng" }, { "family": "Shu", "given": " Fengyue" }, { "family": "Wang", "given": " Haijun" }, { "family": "Ma", "given": " Shuonan" }, { "family": "Li", "given": " Yan" }, { "family": "Jeppesen", "given": " Erik" } ], "container_title": "MICROBIAL ECOLOGY", "id": "258735", "issued": { "date-parts": [ [ 2022, 1, 1 ] ] }, "title": "Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): a Long-Term Pond Experiment", "type": "article-journal" }
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