1 Ocak 2022 Dergi makalesi Açık Erişim

Zhou, Jing; Kong, Yong; Wu, Mengmeng; Shu, Fengyue; Wang, Haijun; Ma, Shuonan; Li, Yan; Jeppesen, Erik

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{
  "DOI": "10.1007/s00248-022-01971-4", 
  "abstract": "Excessive nitrogen (N) input is an important factor influencing aquatic ecosystems and has received increasing public attention in the past decades. It remains unclear how N input affects the denitrifying bacterial communities that play a key role in regulating N cycles in various ecosystems. To test our hypothesis-that the abundance and biodiversity of denitrifying bacterial communities decrease with increasing N-we compared the abundance and composition of denitrifying bacteria having nitrous oxide reductase gene (nosZ I) from sediments (0-20 cm) in five experimental ponds with different nitrogen fertilization treatment (TN10, TN20, TN30, TN40, TN50) using quantitative PCR and pyrosequencing techniques. We found that (1) N addition significantly decreased nosZ I gene abundance, (2) the Invsimpson and Shannon indices (reflecting biodiversity) first increased significantly along with the increasing N loading in TN10-TN40 followed by a decrease in TN50, (3) the beta diversity of the nosZ I denitrifier was clustered into three groups along the TN concentration levels: Cluster I (TN50), Cluster II (TN40), and Cluster III (TN10-TN30), (4) the proportions of Alphaproteobacteria and Betaproteobacteria in the high-N treatment (TN50) were significantly lower than in the lower N treatments (TN10-TN30). (5) The TN concentration was the most important factor driving the alteration of denitrifying bacteria assemblages. Our findings shed new light on the response of denitrification-related bacteria to long-term N loading at pond scale and on the response of denitrifying microorganisms to N pollution.", 
  "author": [
    {
      "family": "Zhou", 
      "given": " Jing"
    }, 
    {
      "family": "Kong", 
      "given": " Yong"
    }, 
    {
      "family": "Wu", 
      "given": " Mengmeng"
    }, 
    {
      "family": "Shu", 
      "given": " Fengyue"
    }, 
    {
      "family": "Wang", 
      "given": " Haijun"
    }, 
    {
      "family": "Ma", 
      "given": " Shuonan"
    }, 
    {
      "family": "Li", 
      "given": " Yan"
    }, 
    {
      "family": "Jeppesen", 
      "given": " Erik"
    }
  ], 
  "container_title": "MICROBIAL ECOLOGY", 
  "id": "258735", 
  "issued": {
    "date-parts": [
      [
        2022, 
        1, 
        1
      ]
    ]
  }, 
  "title": "Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): a Long-Term Pond Experiment", 
  "type": "article-journal"
}