1 Ocak 2021 Dergi makalesi Açık Erişim

Ozdemir Bahadir, Aylin; Balcioglu, Bertan Koray; Serhatli, Muge; Isik, Seyma; Erdag, Berrin

MARC21 XML

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Identifying specific matrix metalloproteinase-2-inhibiting peptides through phage display-based subtractive screening</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">TURKISH JOURNAL OF BIOLOGY</subfield>
    <subfield code="v">45</subfield>
    <subfield code="n">6</subfield>
    <subfield code="c">674-682</subfield>
  </datafield>
  <controlfield tag="001">239884</controlfield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-tubitak-adresli-yayinlar</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">Gelatinases A and B, which are members of the matrix metalloproteinase (MMP) family, play essential roles in cancer development and metastasis, as they can break down basal membranes. Therefore, the determination and inhibition of gelatinases is essential for cancer treatment. Peptides that can specifically block each gelatinase may, therefore, be useful for cancer treatment. In this study, subtractive panning was carried out using a 12-mer peptide library to identify peptides that block gelatinase A activity (MMP-2), which is a key pharmacological target. Using this method, 17 unique peptide sequences were determined. MMP-2 inhibition by these peptides was evaluated through zymogram analyses, which revealed that four peptides inhibited MMP-2 activity by at least 65%. These four peptides were synthesized and used for in vitro wound healing using human umbilical vein endothelial cells, and two peptides, AOMP12 and AOMP29, were found to inhibit wound healing by 40%. These peptides are, thus, potential candidates for MMP2 inhibition for cancer treatment. Furthermore, our findings suggest that our substractive biopanning screening method is a suitable strategy for identifying peptides that selectively inhibit MMP-2.</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="2">opendefinition.org</subfield>
    <subfield code="a">cc-by</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</subfield>
    <subfield code="a">Balcioglu, Bertan Koray</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</subfield>
    <subfield code="a">Serhatli, Muge</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Acibadem Mehmet Ali Aydinlar Univ, Inst Hlth Sci, Dept Med Biotechnol, Istanbul, Turkey</subfield>
    <subfield code="a">Isik, Seyma</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Istanbul Aydin Univ, Dept Med Biol, Basic Med Sci, Is Tanbul, Turkey</subfield>
    <subfield code="a">Erdag, Berrin</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="b">article</subfield>
    <subfield code="a">publication</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</subfield>
    <subfield code="a">Ozdemir Bahadir, Aylin</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2021-01-01</subfield>
  </datafield>
  <controlfield tag="005">20221007104245.0</controlfield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="o">oai:aperta.ulakbim.gov.tr:239884</subfield>
    <subfield code="p">user-tubitak-adresli-yayinlar</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="z">md5:1fd8d238017888ff392760035552b6ec</subfield>
    <subfield code="s">241</subfield>
    <subfield code="u">https://aperta.ulakbim.gov.trrecord/239884/files/bib-d82d367f-77d2-4a2b-8d99-48642bab2fb9.txt</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
    <subfield code="a">Creative Commons Attribution</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.3906/biy-2105-6</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
</record>