1 Ocak 2021 Dergi makalesi Açık Erişim

Ozdemir Bahadir, Aylin; Balcioglu, Bertan Koray; Serhatli, Muge; Isik, Seyma; Erdag, Berrin

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/239884</identifier>
  <creators>
    <creator>
      <creatorName>Ozdemir Bahadir, Aylin</creatorName>
      <givenName>Aylin</givenName>
      <familyName>Ozdemir Bahadir</familyName>
      <affiliation>TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Balcioglu, Bertan Koray</creatorName>
      <givenName>Bertan Koray</givenName>
      <familyName>Balcioglu</familyName>
      <affiliation>TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Serhatli, Muge</creatorName>
      <givenName>Muge</givenName>
      <familyName>Serhatli</familyName>
      <affiliation>TUBITAK, Genet Engn &amp; Biotechnol Inst, Marmara Res Ctr, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Isik, Seyma</creatorName>
      <givenName>Seyma</givenName>
      <familyName>Isik</familyName>
      <affiliation>Acibadem Mehmet Ali Aydinlar Univ, Inst Hlth Sci, Dept Med Biotechnol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erdag, Berrin</creatorName>
      <givenName>Berrin</givenName>
      <familyName>Erdag</familyName>
      <affiliation>Istanbul Aydin Univ, Dept Med Biol, Basic Med Sci, Is Tanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Identifying Specific Matrix Metalloproteinase-2-Inhibiting Peptides Through Phage Display-Based Subtractive Screening</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/239884</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3906/biy-2105-6</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Gelatinases A and B, which are members of the matrix metalloproteinase (MMP) family, play essential roles in cancer development and metastasis, as they can break down basal membranes. Therefore, the determination and inhibition of gelatinases is essential for cancer treatment. Peptides that can specifically block each gelatinase may, therefore, be useful for cancer treatment. In this study, subtractive panning was carried out using a 12-mer peptide library to identify peptides that block gelatinase A activity (MMP-2), which is a key pharmacological target. Using this method, 17 unique peptide sequences were determined. MMP-2 inhibition by these peptides was evaluated through zymogram analyses, which revealed that four peptides inhibited MMP-2 activity by at least 65%. These four peptides were synthesized and used for in vitro wound healing using human umbilical vein endothelial cells, and two peptides, AOMP12 and AOMP29, were found to inhibit wound healing by 40%. These peptides are, thus, potential candidates for MMP2 inhibition for cancer treatment. Furthermore, our findings suggest that our substractive biopanning screening method is a suitable strategy for identifying peptides that selectively inhibit MMP-2.</description>
  </descriptions>
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