1 Ocak 2021 Dergi makalesi Açık Erişim

Yenmis, Guven; Sarac, Elif Yaprak; Besli, Nail; Soydas, Tugba; Tastan, Cihan; Kancagi, Derya Dilek; Yilanci, Muhammet; Senol, Kazim; Karagulle, Onur Olgac; Ekmekci, Cumhur Gokhan; Ovali, Ercument; Tuncdemir, Matem; Ulutin, Turgut; Sultuybek, Gonul Kanigur

MARC21 XML

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Sarac, Elif Yaprak</subfield>
    <subfield code="u">Istanbul Tech Univ, Fac Sci &amp; Letters, Dept Mol Biol Genet &amp; Biotechnol, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Besli, Nail</subfield>
    <subfield code="u">Univ Saglik Bilimleri, Fac Med, Dept Med Biol, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Soydas, Tugba</subfield>
    <subfield code="u">Istanbul Aydin Univ, Fac Med, Dept Med Biol &amp; Genet, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Tastan, Cihan</subfield>
    <subfield code="u">Uskudar Univ, Fac Engn &amp; Nat Sci, Dept Mol Biol &amp; Genet, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Kancagi, Derya Dilek</subfield>
    <subfield code="u">Acibadem Labcell Cell Lab, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Yilanci, Muhammet</subfield>
    <subfield code="u">Acibadem Labcell Cell Lab, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Senol, Kazim</subfield>
    <subfield code="u">Uludag Univ, Fac Med, Dept Breast Surg, Bursa, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Karagulle, Onur Olgac</subfield>
    <subfield code="u">Mimar Sinan State Hosp, Dept Gen Surg, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Ekmekci, Cumhur Gokhan</subfield>
    <subfield code="u">Acibadem Labgen Genet Diagnost Ctr, Acibadem Healthcare Grp, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Ovali, Ercument</subfield>
    <subfield code="u">Acibadem Labcell Cell Lab, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Tuncdemir, Matem</subfield>
    <subfield code="u">Istanbul Univ Cerrahpasa, Fac Med, Dept Med Biol, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Ulutin, Turgut</subfield>
    <subfield code="u">Istanbul Univ Cerrahpasa, Fac Med, Dept Med Biol, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="a">Sultuybek, Gonul Kanigur</subfield>
    <subfield code="u">Istanbul Aydin Univ, Fac Med, Dept Med Biol &amp; Genet, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">ACTA HISTOCHEMICA</subfield>
    <subfield code="v">123</subfield>
    <subfield code="n">4</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="a">Creative Commons Attribution</subfield>
    <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.1016/j.acthis.2021.151709</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Anti-cancer effect of metformin on the metastasis and invasion of primary breast cancer cells through mediating NF-kB activity</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Yenmis, Guven</subfield>
    <subfield code="u">Biruni Univ, Fac Med, Dept Med Biol, Istanbul, Turkey</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="o">oai:aperta.ulakbim.gov.tr:237240</subfield>
    <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="2">opendefinition.org</subfield>
    <subfield code="a">cc-by</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2021-01-01</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="u">https://aperta.ulakbim.gov.trrecord/237240/files/bib-cdb2fdc9-f344-42f6-ae04-b8fb9768e36b.txt</subfield>
    <subfield code="z">md5:4b68a06b56b8ecfa852405c04ff0f583</subfield>
    <subfield code="s">338</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <controlfield tag="005">20221007095132.0</controlfield>
  <controlfield tag="001">237240</controlfield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and 9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). 300 000 cells per ml were obtained from biopsies of breast tumors from five human donors. The cell viability and proliferation were tested. Immunocytochemistry was performed for MMP 2, MMP 9, and NF-kB, and enzyme-linked immunosorbent assay for NF-kB activity, quantitative real-time PCR for RELA/p65, IkBa, MMP-2, and MMP 9. Three different doses of metformin (5, 10, and 25 mM) (Met) reduced the viability and proliferation of PBCCs in a dose-dependent manner, maximum inhibition was observed at 25 mM Met. The expression of RELA/p65 was not affected by 25 mM Met. Nuclear immunoreactivity and activity of NF-kB reduced while cytoplasmic NF-kB (p65) elevated by 25 mM Met compared to non-treatment (P &amp;lt; 0.05). The expression and immunoreactivity of MMP 9 but not MMP 2 were decreased by 25 mM Met treatment, compared with the non-treatment (P &amp;lt; 0.05). Metformin may have an essential antitumor role in the invasion and metastasis pathways of PBCCs by downregulating the MMP 9 expression blocking both the activity and nuclear translocation of NF-kB.</subfield>
  </datafield>
</record>