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Anti-cancer effect of metformin on the metastasis and invasion of primary breast cancer cells through mediating NF-kB activity

Yenmis, Guven; Sarac, Elif Yaprak; Besli, Nail; Soydas, Tugba; Tastan, Cihan; Kancagi, Derya Dilek; Yilanci, Muhammet; Senol, Kazim; Karagulle, Onur Olgac; Ekmekci, Cumhur Gokhan; Ovali, Ercument; Tuncdemir, Matem; Ulutin, Turgut; Sultuybek, Gonul Kanigur


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/237240</identifier>
  <creators>
    <creator>
      <creatorName>Yenmis, Guven</creatorName>
      <givenName>Guven</givenName>
      <familyName>Yenmis</familyName>
      <affiliation>Biruni Univ, Fac Med, Dept Med Biol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sarac, Elif Yaprak</creatorName>
      <givenName>Elif Yaprak</givenName>
      <familyName>Sarac</familyName>
      <affiliation>Istanbul Tech Univ, Fac Sci &amp; Letters, Dept Mol Biol Genet &amp; Biotechnol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Besli, Nail</creatorName>
      <givenName>Nail</givenName>
      <familyName>Besli</familyName>
      <affiliation>Univ Saglik Bilimleri, Fac Med, Dept Med Biol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Soydas, Tugba</creatorName>
      <givenName>Tugba</givenName>
      <familyName>Soydas</familyName>
      <affiliation>Istanbul Aydin Univ, Fac Med, Dept Med Biol &amp; Genet, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tastan, Cihan</creatorName>
      <givenName>Cihan</givenName>
      <familyName>Tastan</familyName>
      <affiliation>Uskudar Univ, Fac Engn &amp; Nat Sci, Dept Mol Biol &amp; Genet, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kancagi, Derya Dilek</creatorName>
      <givenName>Derya Dilek</givenName>
      <familyName>Kancagi</familyName>
      <affiliation>Acibadem Labcell Cell Lab, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yilanci, Muhammet</creatorName>
      <givenName>Muhammet</givenName>
      <familyName>Yilanci</familyName>
      <affiliation>Acibadem Labcell Cell Lab, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Senol, Kazim</creatorName>
      <givenName>Kazim</givenName>
      <familyName>Senol</familyName>
      <affiliation>Uludag Univ, Fac Med, Dept Breast Surg, Bursa, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karagulle, Onur Olgac</creatorName>
      <givenName>Onur Olgac</givenName>
      <familyName>Karagulle</familyName>
      <affiliation>Mimar Sinan State Hosp, Dept Gen Surg, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ekmekci, Cumhur Gokhan</creatorName>
      <givenName>Cumhur Gokhan</givenName>
      <familyName>Ekmekci</familyName>
      <affiliation>Acibadem Labgen Genet Diagnost Ctr, Acibadem Healthcare Grp, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ovali, Ercument</creatorName>
      <givenName>Ercument</givenName>
      <familyName>Ovali</familyName>
      <affiliation>Acibadem Labcell Cell Lab, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tuncdemir, Matem</creatorName>
      <givenName>Matem</givenName>
      <familyName>Tuncdemir</familyName>
      <affiliation>Istanbul Univ Cerrahpasa, Fac Med, Dept Med Biol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ulutin, Turgut</creatorName>
      <givenName>Turgut</givenName>
      <familyName>Ulutin</familyName>
      <affiliation>Istanbul Univ Cerrahpasa, Fac Med, Dept Med Biol, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sultuybek, Gonul Kanigur</creatorName>
      <givenName>Gonul Kanigur</givenName>
      <familyName>Sultuybek</familyName>
      <affiliation>Istanbul Aydin Univ, Fac Med, Dept Med Biol &amp; Genet, Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Anti-Cancer Effect Of Metformin On The Metastasis And Invasion Of Primary Breast Cancer Cells Through Mediating Nf-Kb Activity</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/237240</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.acthis.2021.151709</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Current evidence strongly suggests that aberrant activation of the nuclear factor kappa B (NF-kB) signaling cascade is connected to carcinogenesis. The matrix metalloproteinases (MMP) which are also the key agents for tumor metastasis may be potent candidates for tumor diagnosis in clinics. In this in vitro study, we hypothesized that metformin with an effective dose can inhibit tumor cell proliferation and metastasis by modulating the expressions of MMP-2 and 9 and interfering with NF-kB signaling in primary breast cancer cells (PBCCs). 300 000 cells per ml were obtained from biopsies of breast tumors from five human donors. The cell viability and proliferation were tested. Immunocytochemistry was performed for MMP 2, MMP 9, and NF-kB, and enzyme-linked immunosorbent assay for NF-kB activity, quantitative real-time PCR for RELA/p65, IkBa, MMP-2, and MMP 9. Three different doses of metformin (5, 10, and 25 mM) (Met) reduced the viability and proliferation of PBCCs in a dose-dependent manner, maximum inhibition was observed at 25 mM Met. The expression of RELA/p65 was not affected by 25 mM Met. Nuclear immunoreactivity and activity of NF-kB reduced while cytoplasmic NF-kB (p65) elevated by 25 mM Met compared to non-treatment (P &amp;lt; 0.05). The expression and immunoreactivity of MMP 9 but not MMP 2 were decreased by 25 mM Met treatment, compared with the non-treatment (P &amp;lt; 0.05). Metformin may have an essential antitumor role in the invasion and metastasis pathways of PBCCs by downregulating the MMP 9 expression blocking both the activity and nuclear translocation of NF-kB.</description>
  </descriptions>
</resource>
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