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Comprehensive evaluation of starter culture impact on the bioreactor performance and microbial kinetics

Demirkaya, Esma; Ciftcioglu, Bengisu; Ozyildiz, Goksin; Zengin, Gulsum Emel; Pala-Ozkok, Ilke; Cokgor, Emine; Tas, Didem Okutman


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      {
        "affiliation": "Istanbul Tech Univ, Environm Engn Dept, TR-34469 Maslak, Turkey", 
        "name": "Demirkaya, Esma"
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      {
        "affiliation": "Gebze Tech Univ, Environm Engn Dept, TR-41400 Gebze, Turkey", 
        "name": "Ciftcioglu, Bengisu"
      }, 
      {
        "affiliation": "Istanbul Tech Univ, Environm Engn Dept, TR-34469 Maslak, Turkey", 
        "name": "Ozyildiz, Goksin"
      }, 
      {
        "affiliation": "Istanbul Tech Univ, Environm Engn Dept, TR-34469 Maslak, Turkey", 
        "name": "Zengin, Gulsum Emel"
      }, 
      {
        "affiliation": "Univ Stavanger, Fac Sci & Technol, Inst Chem Biosci & Environm Engn, Box 8600 Forus, N-4036 Stavanger, Norway", 
        "name": "Pala-Ozkok, Ilke"
      }, 
      {
        "affiliation": "Istanbul Tech Univ, Environm Engn Dept, TR-34469 Maslak, Turkey", 
        "name": "Cokgor, Emine"
      }, 
      {
        "affiliation": "Istanbul Tech Univ, Environm Engn Dept, TR-34469 Maslak, Turkey", 
        "name": "Tas, Didem Okutman"
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    ], 
    "description": "The present study investigated the disregarded impact of microbial community composition, diversity and abundance of the starter biomass on lab-scale bioreactor performance, steady-state long time operation, biodegradation outcome and microbial kinetics. Standard lab-scale experiments are usually initiated with inoculum taken from wastewater treatment plants (WWTPs) without considering microbial community composition. Two identical sequencing batch bioreactors (SBR-1, SBR-2) were operated with inocula obtained from two domestic WWTPs to assess the role of inoculum composition on reactor performance and treatment efficacy. Filamentous microorganisms were dominated in SBR-1 (SVI: 265 mL/g), whereas their supremacy was diminished in SBR-2 (SVI: 160 mL/g). Next generation sequencing analyses verified high abundance of Candidatus Microthrix (14%) and Kouleothrix spp. (3%) in SBR-1. Although Chloroflexi C10-SB1A metagenome accounted for 16% of WWTP-2 community, SBR-2 was operated at steady-state for longer period without deterioration. Statistical indicators based on molecular studies showed that WWTP-2 has higher diversity than WWTP-1. Higher biodiversity in WWTP-2 community supported the stable process performance. Modelling studies denoted an increase in half saturation coefficients (K-s) in filamentous microorganisms dominated reactor. The results of this study provide insight on how the filamentous organisms typically found in WWTPs become a fundamental constituent during the bioreactor operation.", 
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      "title": "BIOCHEMICAL ENGINEERING JOURNAL", 
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    "title": "Comprehensive evaluation of starter culture impact on the bioreactor performance and microbial kinetics"
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