Dergi makalesi Açık Erişim

Conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasis

Topuzogullari, Murat; Koc, Rabia Cakir; Isoglu, Sevil Dincer; Bagirova, Melahat; Akdeste, Zeynep; Elcicek, Serhat; Oztel, Olga N.; Baydar, Serap Yesilkir; Ates, Sezen Canim; Allahverdiyev, Adil M.


MARC21 XML

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasis</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">JOURNAL OF BIOMEDICAL SCIENCE</subfield>
    <subfield code="v">20</subfield>
  </datafield>
  <controlfield tag="001">16225</controlfield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">Research on the conjugates of synthetic polyelectrolytes with antigenic molecules, such as proteins, peptides, or carbohydrates, is an attractive area due to their highly immunogenic character in comparison to classical adjuvants. For example, polyacrylic acid (PAA) is a weak polyelectrolyte and has been used in several biomedical applications such as immunological studies, drug delivery, and enzyme immobilization. However, to our knowledge, there are no studies that document immune-stimulant properties of PAA in Leishmania infection. Therefore, we aimed to develop a potential vaccine candidate against leishmaniasis by covalently conjugating PAA with an immunologically vital molecule of lipophosphoglycan (LPG) found in Leishmania parasites. In the study, LPG and PAA were conjugated by a multi-step procedure, and final products were analyzed with GPC and MALDI-TOF MS techniques. In cytotoxicity experiments, LPG-PAA conjugates did not indicate toxic effects on L929 and J774 murine macrophage cells. We assume that LPG-PAA conjugate can be a potential vaccine candidate, and will be immunologically characterized in further studies to prove its potential.</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="2">opendefinition.org</subfield>
    <subfield code="a">cc-by</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yeni Yuzyil Univ, Dept Biomed Engn, Istanbul, Turkey</subfield>
    <subfield code="a">Koc, Rabia Cakir</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Isoglu, Sevil Dincer</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Bagirova, Melahat</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Akdeste, Zeynep</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Firat Univ, Dept Bioengn, TR-23169 Elazig, Turkey</subfield>
    <subfield code="a">Elcicek, Serhat</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Oztel, Olga N.</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Baydar, Serap Yesilkir</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Ates, Sezen Canim</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Allahverdiyev, Adil M.</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="b">article</subfield>
    <subfield code="a">publication</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Yildiz Tech Univ, Dept Bioengn, Istanbul, Turkey</subfield>
    <subfield code="a">Topuzogullari, Murat</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2013-01-01</subfield>
  </datafield>
  <controlfield tag="005">20210315093754.0</controlfield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="o">oai:zenodo.org:16225</subfield>
    <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="z">md5:d3d18d82c9ba571d45fa936bea3c0749</subfield>
    <subfield code="s">310</subfield>
    <subfield code="u">https://aperta.ulakbim.gov.trrecord/16225/files/bib-4a67f826-6aae-497b-80df-ff769eaaea44.txt</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield>
    <subfield code="a">Creative Commons Attribution</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.1186/1423-0127-20-35</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
</record>
23
7
görüntülenme
indirilme
Görüntülenme 23
İndirme 7
Veri hacmi 2.2 kB
Tekil görüntülenme 23
Tekil indirme 7

Alıntı yap