1 Ocak 2020 Dergi makalesi Açık Erişim

Agca, Can Ali; Kirici, Mahinur; Nedzvetsky, Victor S.; Gundogdu, Ramazan; Tykhomyrov, Artem A.

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  <dc:creator>Agca, Can Ali</dc:creator>
  <dc:creator>Kirici, Mahinur</dc:creator>
  <dc:creator>Nedzvetsky, Victor S.</dc:creator>
  <dc:creator>Gundogdu, Ramazan</dc:creator>
  <dc:creator>Tykhomyrov, Artem A.</dc:creator>
  <dc:date>2020-01-01</dc:date>
  <dc:description>Resistance to chemotherapeutic drugs is a critical problem in cancer therapy, but the underlying mechanism has not been fully elucidated. TP53-induced glycolysis regulatory phosphatase (TIGAR), an important glycolysis and apoptosis regulator, plays a crucial role in cancer cell survival by protecting cells against oxidative stress-induced apoptosis. In the present study, we investigated whether TIGAR is involved in epithelial-mesenchymal transition (EMT) in doxorubicin (DOX)-resistant human non-small cell lung cancer (NSCLC), A549/DOX cells. We found that the expression of TIGAR was significantly higher in A549/DOX cells than in the parent A549 cell lines. siRNA-mediated TIGAR knockdown reduced migration, viability and colony survival of doxorubicin-resistant lung cancer cells. Also, TIGAR knockdown decreased pro-survival protein Bcl-2 and increased pro-apoptotic Bax and cleaved poly (ADP-ribose) polymerase (PARP). Moreover, TIGAR depletion significantly up-regulated both caspase-3 and caspase-9 expression. Furthermore, TIGAR depletion up-regulated the expression of E-cadherin and down-regulated the expression of vimentin. These results indicate that TIGAR knockdown may inhibit EMT in doxorubicin (DOX)-resistant human NSCLC and may represent a therapeutic target for a non-small lung cancer cells chemoresistance.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/10957</dc:identifier>
  <dc:identifier>oai:zenodo.org:10957</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>CHEMISTRY &amp; BIODIVERSITY 17(9)</dc:source>
  <dc:title>The Effect of TIGAR Knockdown on Apoptotic and Epithelial-Mesenchymal Markers Expression in Doxorubicin-Resistant Non-Small Cell Lung Cancer A549 Cell Lines</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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