1 Ocak 2020 Dergi makalesi Açık Erişim

Agca, Can Ali; Kirici, Mahinur; Nedzvetsky, Victor S.; Gundogdu, Ramazan; Tykhomyrov, Artem A.

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/10957</identifier>
  <creators>
    <creator>
      <creatorName>Agca, Can Ali</creatorName>
      <givenName>Can Ali</givenName>
      <familyName>Agca</familyName>
      <affiliation>Bingol Univ, Dept Mol Biol &amp; Genet, TR-12000 Bingol, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kirici, Mahinur</creatorName>
      <givenName>Mahinur</givenName>
      <familyName>Kirici</familyName>
      <affiliation>Bingol Univ, Dept Chem, TR-12000 Bingol, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Nedzvetsky, Victor S.</creatorName>
      <givenName>Victor S.</givenName>
      <familyName>Nedzvetsky</familyName>
    </creator>
    <creator>
      <creatorName>Gundogdu, Ramazan</creatorName>
      <givenName>Ramazan</givenName>
      <familyName>Gundogdu</familyName>
      <affiliation>Bingol Univ, Dept Biol, TR-12000 Bingol, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tykhomyrov, Artem A.</creatorName>
      <givenName>Artem A.</givenName>
      <familyName>Tykhomyrov</familyName>
      <affiliation>Natl Acad Sci Ukraine, Dept Enzyme Chem &amp; Biochem, Palladin Inst Biochem, UA-01030 Kiev, Ukraine</affiliation>
    </creator>
  </creators>
  <titles>
    <title>The Effect Of Tigar Knockdown On Apoptotic And Epithelial-Mesenchymal Markers Expression In Doxorubicin-Resistant Non-Small Cell Lung Cancer A549 Cell Lines</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2020</publicationYear>
  <dates>
    <date dateType="Issued">2020-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/10957</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1002/cbdv.202000441</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Resistance to chemotherapeutic drugs is a critical problem in cancer therapy, but the underlying mechanism has not been fully elucidated. TP53-induced glycolysis regulatory phosphatase (TIGAR), an important glycolysis and apoptosis regulator, plays a crucial role in cancer cell survival by protecting cells against oxidative stress-induced apoptosis. In the present study, we investigated whether TIGAR is involved in epithelial-mesenchymal transition (EMT) in doxorubicin (DOX)-resistant human non-small cell lung cancer (NSCLC), A549/DOX cells. We found that the expression of TIGAR was significantly higher in A549/DOX cells than in the parent A549 cell lines. siRNA-mediated TIGAR knockdown reduced migration, viability and colony survival of doxorubicin-resistant lung cancer cells. Also, TIGAR knockdown decreased pro-survival protein Bcl-2 and increased pro-apoptotic Bax and cleaved poly (ADP-ribose) polymerase (PARP). Moreover, TIGAR depletion significantly up-regulated both caspase-3 and caspase-9 expression. Furthermore, TIGAR depletion up-regulated the expression of E-cadherin and down-regulated the expression of vimentin. These results indicate that TIGAR knockdown may inhibit EMT in doxorubicin (DOX)-resistant human NSCLC and may represent a therapeutic target for a non-small lung cancer cells chemoresistance.</description>
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