Dergi makalesi Açık Erişim
Reeder, Sophia M.; Reuschel, Emma L.; Bah, Mamadou A.; Yun, Kun; Tursi, Nicholas J.; Kim, Kevin Y.; Chu, Jacqueline; Zaidi, Faraz, I; Yilmaz, Ilknur; Hart, Robert J.; Perrin, Benjamin; Xu, Ziyang; Humeau, Laurent; Weiner, David B.; Aly, Ahmed S., I
<?xml version='1.0' encoding='UTF-8'?> <record xmlns="http://www.loc.gov/MARC21/slim"> <leader>00000nam##2200000uu#4500</leader> <datafield tag="245" ind1=" " ind2=" "> <subfield code="a">Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model</subfield> </datafield> <datafield tag="909" ind1="C" ind2="4"> <subfield code="p">VACCINES</subfield> <subfield code="v">8</subfield> <subfield code="n">1</subfield> </datafield> <controlfield tag="001">10305</controlfield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="520" ind1=" " ind2=" "> <subfield code="a">The need for a malaria vaccine is indisputable. A single vaccine for Plasmodium pre-erythrocytic stages targeting the major sporozoite antigen circumsporozoite protein (CSP) has had partial success. Additionally, CD8+ T cells targeting liver-stage (LS) antigens induced by live attenuated sporozoite vaccines were associated with protection in human challenge experiments. To further evaluate protection mediated by LS antigens, we focused on exported pre-erythrocytic proteins (exported protein 1 (EXP1), profilin (PFN), exported protein 2 (EXP2), inhibitor of cysteine proteases (ICP), transmembrane protein 21 (TMP21), and upregulated in infective sporozoites-3 (UIS3)) expressed in all Plasmodium species and designed optimized, synthetic DNA (synDNA) immunogens. SynDNA antigen cocktails were tested with and without the molecular adjuvant plasmid IL-33. Immunized animals developed robust T cell responses including induction of antigen-specific liver-localized CD8+ T cells, which were enhanced by the co-delivery of plasmid IL-33. In total, 100% of mice in adjuvanted groups and 71%-88% in non-adjuvanted groups were protected from blood-stage disease following Plasmodium yoelii sporozoite challenge. This study supports the potential of synDNA LS antigens as vaccine components for malaria parasite infection.</subfield> </datafield> <datafield tag="650" ind1="1" ind2="7"> <subfield code="2">opendefinition.org</subfield> <subfield code="a">cc-by</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Reuschel, Emma L.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Bah, Mamadou A.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Yun, Kun</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Tursi, Nicholas J.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Kim, Kevin Y.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Chu, Jacqueline</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Zaidi, Faraz, I</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Bezmialem Vakif Univ, Beykoz Inst Life Sci & Biotechnol, Istanbul 34820, Turkey</subfield> <subfield code="a">Yilmaz, Ilknur</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Tulane Univ, Dept Trop Med, New Orleans, LA 70112 USA</subfield> <subfield code="a">Hart, Robert J.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Tulane Univ, Dept Trop Med, New Orleans, LA 70112 USA</subfield> <subfield code="a">Perrin, Benjamin</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Xu, Ziyang</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Inovio Pharmaceut Inc, Plymouth Meeting, PA 19462 USA</subfield> <subfield code="a">Humeau, Laurent</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">Vaccine Ctr, Wistar Inst, Philadelphia, PA 19104 USA</subfield> <subfield code="a">Weiner, David B.</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Aly, Ahmed S., I</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="b">article</subfield> <subfield code="a">publication</subfield> </datafield> <datafield tag="542" ind1=" " ind2=" "> <subfield code="l">open</subfield> </datafield> <datafield tag="100" ind1=" " ind2=" "> <subfield code="a">Reeder, Sophia M.</subfield> </datafield> <datafield tag="260" ind1=" " ind2=" "> <subfield code="c">2020-01-01</subfield> </datafield> <controlfield tag="005">20210315072501.0</controlfield> <datafield tag="909" ind1="C" ind2="O"> <subfield code="o">oai:zenodo.org:10305</subfield> <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="856" ind1="4" ind2=" "> <subfield code="z">md5:c628bc398cb8f62afed821ffd99525e1</subfield> <subfield code="s">325</subfield> <subfield code="u">https://aperta.ulakbim.gov.trrecord/10305/files/bib-3bb959c7-3123-4db1-9158-2bf0136cfb96.txt</subfield> </datafield> <datafield tag="540" ind1=" " ind2=" "> <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield> <subfield code="a">Creative Commons Attribution</subfield> </datafield> <datafield tag="024" ind1=" " ind2=" "> <subfield code="a">10.3390/vaccines8010021</subfield> <subfield code="2">doi</subfield> </datafield> </record>
Görüntülenme | 46 |
İndirme | 5 |
Veri hacmi | 1.6 kB |
Tekil görüntülenme | 44 |
Tekil indirme | 5 |