1 Ocak 2005 Dergi makalesi Açık Erişim

Gokmen, V; Senyuva, HZ; Acar, J; Sarioglu, K

Citation Style Language JSON

{
  "DOI": "10.1016/j.chroma.2004.10.094", 
  "abstract": "A simple and rapid method using liquid chromatography coupled to diode array detection (LC-DAD) was developed for the determination of acrylamide in potato-based foods at low levels. The method entails extraction of acrylamide with methanol, purification with Carrez I and 11 solutions, evaporation and solvent change to water, and cleanup with a Oasis HLB solid-phase extraction (SPE) cartridge. The final extract was analyzed by LC-DAD for quantification and by liquid chromatography coupled to mass spectrometry (LC-MS) for confirmation. The chromatographic separations were performed on a hydrophilic and a hydrophobic interaction columns having good retention of acrylamide under 100% aqueous flow conditions (k' 3.67 and 2.54, respectively). The limit of quantitation was estimated to be 4.0 mu g/kg based on the signal-to-noise ratio of 3 recorded at 226 nm. Recoveries of acrylamide from potato chips samples spiked at levels of 250, 500 and 1000 (n = 4 for each level) mu g/kg ranged between 92.8 and 96.2% with relative standard deviations of less than 5%. The results of this study revealed that a conventional LC instrument coupled to DAD can also be used accurately and precisely, as an alternative to tandem LC-MS methods for the determination of acrylamide in potato-based foods. (c) 2004 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Gokmen", 
      "given": " V"
    }, 
    {
      "family": "Senyuva", 
      "given": " HZ"
    }, 
    {
      "family": "Acar", 
      "given": " J"
    }, 
    {
      "family": "Sarioglu", 
      "given": " K"
    }
  ], 
  "container_title": "JOURNAL OF CHROMATOGRAPHY A", 
  "id": "95055", 
  "issue": "1-2", 
  "issued": {
    "date-parts": [
      [
        2005, 
        1, 
        1
      ]
    ]
  }, 
  "page": "193-199", 
  "title": "Determination of acrylamide in potato chips and crisps by high-performance liquid chromatography", 
  "type": "article-journal", 
  "volume": "1088"
}