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Detection of cystine and cysteine in wheat flour using a robust LC-MS/MS method

Dogan, Canan Ekinci; Cebi, Nur; Develioglu, Aysen; Olgun, Elmas Oktem; Sagdic, Osman


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{
  "@context": "https://schema.org/", 
  "@id": 93227, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "TUBITAK MRC Food Inst, TR-41470 Gebze, Kocaeli, Turkey", 
      "name": "Dogan, Canan Ekinci"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yildiz Tech Univ, Chem & Met Engn Fac, Dept Food Engn, TR-34210 Istanbul, Turkey", 
      "name": "Cebi, Nur"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yildiz Tech Univ, Chem & Met Engn Fac, Dept Food Engn, TR-34210 Istanbul, Turkey", 
      "name": "Develioglu, Aysen"
    }, 
    {
      "@type": "Person", 
      "affiliation": "TUBITAK MRC Food Inst, TR-41470 Gebze, Kocaeli, Turkey", 
      "name": "Olgun, Elmas Oktem"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yildiz Tech Univ, Chem & Met Engn Fac, Dept Food Engn, TR-34210 Istanbul, Turkey", 
      "name": "Sagdic, Osman"
    }
  ], 
  "datePublished": "2018-01-01", 
  "description": "An effective and simultaneous liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the first time, with the aim of quantification of cysteine and cystine in wheat flour. Developed LC-MS/MS method showed good linearity (R-2 = 0.99) with very low LOD and LOQ values as 2.0 mu g/kg and 7.0 mu g/kg for cystine, 3.0 mu g/kg and 8.0 mu g/kg for cysteine, respectively. Favorable repeatability and reproducibility were achieved. Developed highly sensitive LC-MS/MS method was successfully applied for the detection and quantification of cystine and cysteine in wheat flour samples. Cystine and cysteine contents were determined as quite similar and appropriate for 25 types of wheat flour. In addition, method has great capability for quantification of additional cysteine and cystine in the wheat flour since quite favorable recovery values were obtained.", 
  "headline": "Detection of cystine and cysteine in wheat flour using a robust LC-MS/MS method", 
  "identifier": 93227, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Detection of cystine and cysteine in wheat flour using a robust LC-MS/MS method", 
  "url": "https://aperta.ulakbim.gov.tr/record/93227"
}
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