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Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

Kilinc, Fatih Mehmet; Suzerer, Veysel; Ciftci, Yelda Ozden; Onay, Ahmet; Yildirim, Hakan; Uncuoglu, Ahu Altinkut; Tilkat, Engin; Koc, Ibrahim; Akdemir, Omer Faruk; Metin, Ozge Karakas


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    "creators": [
      {
        "affiliation": "Dicle Univ, Dept Biol, Fac Sci, TR-21280 Diyarbakir, Turkey", 
        "name": "Kilinc, Fatih Mehmet"
      }, 
      {
        "affiliation": "Bingol Univ, Vocat Sch Hlth Serv, Med Lab, TR-12000 Bingol, Turkey", 
        "name": "Suzerer, Veysel"
      }, 
      {
        "affiliation": "Gebze Inst Technol, Dept Mol Biol & Genet, TR-41400 Gebze, Kocaeli, Turkey", 
        "name": "Ciftci, Yelda Ozden"
      }, 
      {
        "affiliation": "Dicle Univ, Dept Biol, Fac Sci, TR-21280 Diyarbakir, Turkey", 
        "name": "Onay, Ahmet"
      }, 
      {
        "affiliation": "Dicle Univ, Dept Hort, Fac Agr, TR-21280 Diyarbakir, Turkey", 
        "name": "Yildirim, Hakan"
      }, 
      {
        "affiliation": "Marmara Univ, Dept Bioengn, Fac Engn, TR-34722 Istanbul, Turkey", 
        "name": "Uncuoglu, Ahu Altinkut"
      }, 
      {
        "affiliation": "Batman Univ, Fac Sci & Literature, Dept Biol, TR-72100 Batman, Turkey", 
        "name": "Tilkat, Engin"
      }, 
      {
        "affiliation": "Gebze Inst Technol, Dept Mol Biol & Genet, TR-41400 Gebze, Kocaeli, Turkey", 
        "name": "Koc, Ibrahim"
      }, 
      {
        "affiliation": "Dicle Univ, Dept Biol, Fac Sci, TR-21280 Diyarbakir, Turkey", 
        "name": "Akdemir, Omer Faruk"
      }, 
      {
        "affiliation": "TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey", 
        "name": "Metin, Ozge Karakas"
      }
    ], 
    "description": "An efficient protocol for clonal micropropagation of selected genotypes of lentisk, Pistacia lentiscus L., which is cultivated for the masticha resin, has been developed using shoot tip explants originating from in vitro seedlings. BA was found to be optimum for shoot morphogenesis in terms of the number and length of shoots among the cytokinins tested for all cloned genotypes, while the highest shoot length was noticed in the presence of 2iP at a rate 4.92 mu M. Efficient rooting (94.15 %) was achieved in a medium containing 19.6 mu M IBA with the clone II that was superior to the rest of the clones tested. The method developed for plant acclimatization was satisfactory because a high percentage of plant survival (95 %) in the growth room in the clone II was obtained and the regenerated plantlets resumed growth after 4 months. DNAs from mother seedlings and micropropagated plantlets belonging to 6, 9 and 12 times subcultured were isolated and subjected to IRAP analysis in order to evaluate their genetic stability and detect possibly existing variations among in vitro derived plantlets. The mean percentage of similarity calculated by Jaccard's similarity coefficient ranged from 78 to 86 % in the four genotypes. Although variation was observed among mother plantlets and its regenerants for all of the clones, polymorphic information content value in the range of 0.391-0.405 indicated the presence of reasonable polymorphism within the clones. The presented data confirmed that the clonal propagation of lentisk by using shoot tips could be used for commercial exploitation of the selected genotype.", 
    "doi": "10.1007/s10725-014-9933-9", 
    "has_grant": false, 
    "journal": {
      "issue": "1", 
      "pages": "75-88", 
      "title": "PLANT GROWTH REGULATION", 
      "volume": "75"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2015-01-01", 
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    "title": "Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers"
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