1 Ocak 2009 Dergi makalesi Açık Erişim

Oeztuerk, Selcuk; Oezeren-Morgan, Mueserref; Dilgimen, Aydan Salman; Denizci, Aziz Akin; Arikan, Burhan; Kazan, Dilek

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/89969</identifier>
  <creators>
    <creator>
      <creatorName>Oeztuerk, Selcuk</creatorName>
      <givenName>Selcuk</givenName>
      <familyName>Oeztuerk</familyName>
      <affiliation>Marmara Univ, Fac Arts &amp; Sci, Dept Biol, TR-81040 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Oezeren-Morgan, Mueserref</creatorName>
      <givenName>Mueserref</givenName>
      <familyName>Oezeren-Morgan</familyName>
      <affiliation>Marmara Univ, Fac Arts &amp; Sci, Dept Biol, TR-81040 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Dilgimen, Aydan Salman</creatorName>
      <givenName>Aydan Salman</givenName>
      <familyName>Dilgimen</familyName>
      <affiliation>Marmara Univ, Fac Engn, Dept Bioengn, TR-34722 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Denizci, Aziz Akin</creatorName>
      <givenName>Aziz Akin</givenName>
      <familyName>Denizci</familyName>
      <affiliation>MRC, GEBI, Sci Technol Res Council Turkey TUBITAK, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Arikan, Burhan</creatorName>
      <givenName>Burhan</givenName>
      <familyName>Arikan</familyName>
      <affiliation>Cukurova Univ, Fac Art &amp; Sci, Dept Biol, Adana, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kazan, Dilek</creatorName>
      <givenName>Dilek</givenName>
      <familyName>Kazan</familyName>
    </creator>
  </creators>
  <titles>
    <title>Alkaline Serine Protease From Halatoerant Bacillus Licheniformis Ba17</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2009</publicationYear>
  <dates>
    <date dateType="Issued">2009-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/89969</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.89968</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.89969</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">An alkaline protease from halotolerant Bacillus licheniformis BA17, isolated from Van Lake in Turkey, was purified 5.4 fold with 58% yield. The molecular weight was 19.7 kDa and the optimum temperature and pH were 60 degrees C and 10, respectively. The half-life of the pure enzyme was 38 h, 93 min, 14 min and 6 min at 40, 50, 60 and 70 degrees C, respectively. BA17 protease is very active at 30 degrees C between pH 8.0 and 10. Enzyme activity increased in the presence of Cu+2, Mg+2, Mn+2 and K+1 ions, Enzyme retained activity with 5% SDS (w/v) and 1% Triton X-100 (v/v). Inhibition with PMSF and EDTA suggested that the enzyme is a serine protease and is a metal-activated enzyme. Based on the N-terminal sequence of the first 13 amino acids, B. licheniformis BA17 alkaline protease did not show identity to any of those from other Bacillus species.</description>
  </descriptions>
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