1 Ocak 2011 Dergi makalesi Açık Erişim

Sogut, Ibrahim; Hatipoglu, Ibrahim; Serhatli, Muge; Cevik, Safak Isil; Durali, Deniz; Basalp, Aynur

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/89559</identifier>
  <creators>
    <creator>
      <creatorName>Sogut, Ibrahim</creatorName>
      <givenName>Ibrahim</givenName>
      <familyName>Sogut</familyName>
      <affiliation>TUBITAK Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Hatipoglu, Ibrahim</creatorName>
      <givenName>Ibrahim</givenName>
      <familyName>Hatipoglu</familyName>
      <affiliation>TUBITAK Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Serhatli, Muge</creatorName>
      <givenName>Muge</givenName>
      <familyName>Serhatli</familyName>
      <affiliation>TUBITAK Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Cevik, Safak Isil</creatorName>
      <givenName>Safak Isil</givenName>
      <familyName>Cevik</familyName>
      <affiliation>Sabanci Univ Orhanli, Biol Sci &amp; Bioengn Program, Fac Engn &amp; Nat Sci, TR-34956 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Durali, Deniz</creatorName>
      <givenName>Deniz</givenName>
      <familyName>Durali</familyName>
      <affiliation>TUBITAK Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Basalp, Aynur</creatorName>
      <givenName>Aynur</givenName>
      <familyName>Basalp</familyName>
      <affiliation>TUBITAK Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Cryopreservation Of Spleen And Lymph Nodes As A Source Of Mononuclear Cells To Be Used For The Development Of Monoclonal Antibody Producing Hybridoma Cells</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2011</publicationYear>
  <dates>
    <date dateType="Issued">2011-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/89559</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.89558</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.89559</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In the present study, spleen and lymph nodes of mice were cryopreserved as a whole tissue and after thawing, membrane integrity of mononuclear cells was determined by trypan blue exclusion and PI staining. T and B lymphocytes, macrophages and dendritic cells have been isolated from both cryopreserved tissue and analyzed by Flow cytometry. BALB/c mice were immunized with Hepatitis e antigen (HBeAg) and spleen and lymph nodes of mice were cryopreserved for 3 to 10 months. The cells obtained from both tissue were applied to hybridoma technology to understand if the cells keep their viability and functionality. The cells were isolated and fused with F0 mouse myeloma cells and several antibody producing hybrid cells were developed. Results have shown that cryopreserved spleen and lymph nodes of mice can be efficiently used in hybridoma technology for the successful generation of monoclonal antibody producing hybrid cells.</description>
  </descriptions>
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