1 Ocak 2011 Dergi makalesi Açık Erişim

Bahadir, Aylin Ozdemir; Balcioglu, Bertan Koray; Uzyol, Kamil Serkan; Hatipoglu, Ibrahim; Sogut, Ibrahim; Basalp, Aynur; Erdag, Berrin

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/89419</identifier>
  <creators>
    <creator>
      <creatorName>Bahadir, Aylin Ozdemir</creatorName>
      <givenName>Aylin Ozdemir</givenName>
      <familyName>Bahadir</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Balcioglu, Bertan Koray</creatorName>
      <givenName>Bertan Koray</givenName>
      <familyName>Balcioglu</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Uzyol, Kamil Serkan</creatorName>
      <givenName>Kamil Serkan</givenName>
      <familyName>Uzyol</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Hatipoglu, Ibrahim</creatorName>
      <givenName>Ibrahim</givenName>
      <familyName>Hatipoglu</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sogut, Ibrahim</creatorName>
      <givenName>Ibrahim</givenName>
      <familyName>Sogut</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Basalp, Aynur</creatorName>
      <givenName>Aynur</givenName>
      <familyName>Basalp</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Erdag, Berrin</creatorName>
      <givenName>Berrin</givenName>
      <familyName>Erdag</familyName>
      <affiliation>Genet Engn &amp; Biotechnol Inst, Tubitak Marmara Res Ctr, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Phage Displayed Hbv Core Antigen With Immunogenic Activity</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2011</publicationYear>
  <dates>
    <date dateType="Issued">2011-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/89419</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s12010-011-9365-1</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.</description>
  </descriptions>
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