1 Ocak 2020 Dergi makalesi Açık Erişim

Gulyuz, Sevgi; Bayram, Duygu; Ozkose, Umut Ugur; Bolat, Zeynep Busra; Kocak, Polen; Saka, Ongun Mehmet; Devrim, Burcu; Khalily, Melek Parlak; Telci, Dilek; Sahin, Fikrettin; Ozcubukcu, Salih; Sezer, Esma; Tasdelen, Mehmet Atilla; Alpturk, Onur; Bozkir, Asuman; Yilmaz, Ozgur

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{
  "@context": "https://schema.org/", 
  "@id": 89227, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Gulyuz, Sevgi"
    }, 
    {
      "@type": "Person", 
      "name": "Bayram, Duygu"
    }, 
    {
      "@type": "Person", 
      "name": "Ozkose, Umut Ugur"
    }, 
    {
      "@type": "Person", 
      "name": "Bolat, Zeynep Busra"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, Istanbul, Turkey", 
      "name": "Kocak, Polen"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkey", 
      "name": "Saka, Ongun Mehmet"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkey", 
      "name": "Devrim, Burcu"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yozgat Bozok Univ, Fac Arts & Sci, Dept Chem, Yozgat, Turkey", 
      "name": "Khalily, Melek Parlak"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, Istanbul, Turkey", 
      "name": "Telci, Dilek"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yeditepe Univ, Fac Engn & Architecture, Dept Genet & Bioengn, Istanbul, Turkey", 
      "name": "Sahin, Fikrettin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Middle East Tech Univ, Fac Arts & Sci, Dept Chem, Ankara, Turkey", 
      "name": "Ozcubukcu, Salih"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Istanbul Tech Univ, Fac Sci & Letters, Dept Chem, Istanbul, Turkey", 
      "name": "Sezer, Esma"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Yalova Univ, Fac Engn, Dept Polymer Engn, Yalova, Turkey", 
      "name": "Tasdelen, Mehmet Atilla"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Istanbul Tech Univ, Fac Sci & Letters, Dept Chem, Istanbul, Turkey", 
      "name": "Alpturk, Onur"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkey", 
      "name": "Bozkir, Asuman"
    }, 
    {
      "@type": "Person", 
      "affiliation": "TUBITAK, Marmara Res Ctr, Mat Inst, Kocaeli, Turkey", 
      "name": "Yilmaz, Ozgur"
    }
  ], 
  "datePublished": "2020-01-01", 
  "description": "Liposome surface modifications serve great potential applications of liposomes, for instance, increasing stability, bioactive liposome conjugates, and targeted drug, gene, and image agent delivery. In this study, novel targeted lipopolymers, peptide 18/peptide 563-poly(2-ethyl-2-oxazoline)-dioleoylphosphatidyl-ethanolamine (P18/P563-PEtOx-DOPE), have been demonstrated to be successfully synthesized. The structures of P18/P563-PEtOx-DOPE were confirmed by FT-IR spectroscopy, GPC, and(1)H-NMR. In this strategy, poly(2-ethyl 2-oxazoline)-modified liposomes were firstly constructed with molecular weights of 3,500 and 5,800 Da. Then, we chose PEtOx(5800)-DOPE because it has been obtained better particle size (88.74 +/- 0.6816) according to the DLS results. Then, peptides- and dye-PEtOx lipid-based nanovesicle (LN) were prepared by peptide-18, peptide-563, and 7-mercapto-4-methyl coumarin. Genetic material (pDNA) was encapsulated into the liposomes and evaluated the encapsulation of plasmid DNA with migration by using agarose gel electrophoresis.In vitrocytotoxicity experiment results on prostate cancer and breast cancer cell lines, parallelly with the healthy prostate (PNT1A) and breast (MCF10A) epithelial cell lines, cells showed insignificant toxic effects. Thus, we can suggest a novel PEtOx phospholipid thanks to this article and its integration with ligands, which great potential for gene transfer system.", 
  "headline": "Synthesis, biocompatibility and gene encapsulation of poly(2-Ethyl 2-Oxazoline)-dioleoyl phosphatidylethanolamine (PEtOx-DOPE) and post-modifications with peptides and fluorescent dye coumarin", 
  "identifier": 89227, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Synthesis, biocompatibility and gene encapsulation of poly(2-Ethyl 2-Oxazoline)-dioleoyl phosphatidylethanolamine (PEtOx-DOPE) and post-modifications with peptides and fluorescent dye coumarin", 
  "url": "https://aperta.ulakbim.gov.tr/record/89227"
}