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Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery

2020    Gokce, Zeliha Guler; Birol, Semra Zuhal; Mitina, Nataliya; Harhay, Khrystyna; Finiuk, Nataliya; Glasunova, Valentina; Stoika, Rostyslav; Ercelen, Sebnem; Zaichenko, Alexander

Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Strong compaction of the DNA upon interaction with polymer at CMC value, positive charge, and high stability of the polyplex are key factors promoting the penetration of DNA through bio-surfaces that define the efficiency of gene delivery in mammalian cells.