1 Ocak 2012 Dergi makalesi Açık Erişim

Yildiz-Unal, Aysegul; Korulu, Sirin; Karabay, Arzu

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/87281</identifier>
  <creators>
    <creator>
      <creatorName>Yildiz-Unal, Aysegul</creatorName>
      <givenName>Aysegul</givenName>
      <familyName>Yildiz-Unal</familyName>
    </creator>
    <creator>
      <creatorName>Korulu, Sirin</creatorName>
      <givenName>Sirin</givenName>
      <familyName>Korulu</familyName>
    </creator>
    <creator>
      <creatorName>Karabay, Arzu</creatorName>
      <givenName>Arzu</givenName>
      <familyName>Karabay</familyName>
      <affiliation>Istanbul Tech Univ, Dept Mol Biol &amp; Genet, Fac Sci &amp; Letters, TR-34469 Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Q111Qc Vspeedyringo Inhibits Calpain-Directed Apoptosis In Neurons</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2012</publicationYear>
  <dates>
    <date dateType="Issued">2012-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/87281</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3233/JAD-2012-120652</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
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  <descriptions>
    <description descriptionType="Abstract">The calcium-activated proteolytic enzyme calpain is one of the key proteins that can directly or indirectly drive neurons into apoptosis. The indirect way is through cyclin dependent kinase 5 (CDK5), a non-mitotic kinase, which is upregulated through calpain overactivation and followed by a subsequent increase in p53 and active caspase-3 levels under neurodegenerative conditions. The direct way is the upregulation of p53 by calpain itself, since p53 is a substrate for it. SpeedyRINGO is an atypical cell cycle regulator that has been shown to have protective effects in mitotic cells against apoptosis by inhibiting caspase-3 activation when p53 is present. Our aim was to reveal possible protective effects of SpeedyRINGO against calpain-induced caspase-3 activation in neurons which is crucial in terms of providing novel insights in preventing the caspase-3 activation cascade in neurodegeneration. For this reason, mRNA and protein levels were analyzed by qRT-PCR, western blotting, and immunofluorescence. We show that calpain overactivation leads to the upregulation of p53 and a subsequent increase in active caspase-3 level, indicating activation of apoptotic machinery in neurons. This calpain-directed caspase-3 activation upon upregulation of p53 is inhibited by the expression of SpeedyRINGO in rat hippocampal neurons. Therefore, SpeedyRINGO acts as a savior for neurons that are under apoptotisis due to caspase-3 activation.</description>
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