1 Ocak 2020 Dergi makalesi Açık Erişim

Deveci Ozkan, Asuman; Kaleli, Suleyman; Onen, Hacer Ilke; Sarihan, Mehmet; Guney Eskiler, Gamze; Kalayci Yigin, Aysel; Akdogan, Mehmet

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  <dc:creator>Deveci Ozkan, Asuman</dc:creator>
  <dc:creator>Kaleli, Suleyman</dc:creator>
  <dc:creator>Onen, Hacer Ilke</dc:creator>
  <dc:creator>Sarihan, Mehmet</dc:creator>
  <dc:creator>Guney Eskiler, Gamze</dc:creator>
  <dc:creator>Kalayci Yigin, Aysel</dc:creator>
  <dc:creator>Akdogan, Mehmet</dc:creator>
  <dc:date>2020-01-01</dc:date>
  <dc:description>Background: Toll-like receptors (TLRs) are often expressed in natural immune cells as well as in tumor cells. TLR4 exhibits both tumor promoting and tumor-suppressing roles and higher TLR9 expression is an important marker of poor prognosis in prostate cancer (PCa). Nobiletin (NOB) is an O-methylated flavonoid and NOB has been proven to have anti-cancer effect in PCa cells. However, there is no study in the literature investigating the potential anti-inflammatory effects of NOB on the TLR signaling pathways in cancer. Therefore, we aimed to explore the potential anti-inflammatory effects of NOB on the TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in different types of PCa cell lines, for the first time. Material and methods: In the current study, the cytotoxic effect of NOB PC-3 (hormone-independent and metastatic) and LNCaP cells (hormone-dependent) was evaluated by WST-1 assay. Furthermore, the inhibitory effects of NOB on TLR4/TRIF/IRF3 and TLR9/IRF7signaling pathway were determined by RT-PCR, western blotting and ELISA analysis. Results: NOB demonstrated an inhibitory effect on PCa cell growth and LNCaP cells were more sensitive to NOB than PC-3 cells due to androjen receptor status. Furthermore, NOB alone could suppress TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways through the downregulation of their associated pathways (mRNA and related protein levels) and the release of IFN-alpha and IFN-beta compared to LPS or CpG-ODN stimulated PCa cells. Conclusions: NOB potentially inhibited TLR4 and TL9-dependent signaling pathway in PCa cells. However, the efficacy of NOB was different in PCa cells due to the hormone status and aggressive features.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/8489</dc:identifier>
  <dc:identifier>oai:zenodo.org:8489</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY 42(2) 93-100</dc:source>
  <dc:title>Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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