1 Ocak 2015 Dergi makalesi Açık Erişim

Ozden, Sibel; Kara, Neslihan Turgut; Sezerman, Osman Ugur; Durasi, Ilknur Melis; Chen, Tao; Demirel, Goksun; Alpertunga, Buket; Chipman, J. Kevin; Mally, Angela

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/82301</identifier>
  <creators>
    <creator>
      <creatorName>Ozden, Sibel</creatorName>
      <givenName>Sibel</givenName>
      <familyName>Ozden</familyName>
      <affiliation>Istanbul Univ, Dept Pharmaceut Toxicol, Fac Pharm, TR-34116 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kara, Neslihan Turgut</creatorName>
      <givenName>Neslihan Turgut</givenName>
      <familyName>Kara</familyName>
      <affiliation>Istanbul Univ, Fac Sci, Dept Mol Biol &amp; Genet, TR-34116 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sezerman, Osman Ugur</creatorName>
      <givenName>Osman Ugur</givenName>
      <familyName>Sezerman</familyName>
      <affiliation>Acibadem Univ, Dept Biostat &amp; Med Informat, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Durasi, Ilknur Melis</creatorName>
      <givenName>Ilknur Melis</givenName>
      <familyName>Durasi</familyName>
      <affiliation>Sabanci Univ, Fac Engn &amp; Nat Sci, Biol Sci &amp; Bioengn, Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Chen, Tao</creatorName>
      <givenName>Tao</givenName>
      <familyName>Chen</familyName>
      <affiliation>Soochow Univ, Sch Publ Hlth, Dept Toxicol, Suzhou, Peoples R China</affiliation>
    </creator>
    <creator>
      <creatorName>Demirel, Goksun</creatorName>
      <givenName>Goksun</givenName>
      <familyName>Demirel</familyName>
      <affiliation>Istanbul Univ, Dept Pharmaceut Toxicol, Fac Pharm, TR-34116 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Alpertunga, Buket</creatorName>
      <givenName>Buket</givenName>
      <familyName>Alpertunga</familyName>
      <affiliation>Istanbul Univ, Dept Pharmaceut Toxicol, Fac Pharm, TR-34116 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Chipman, J. Kevin</creatorName>
      <givenName>J. Kevin</givenName>
      <familyName>Chipman</familyName>
      <affiliation>Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England</affiliation>
    </creator>
    <creator>
      <creatorName>Mally, Angela</creatorName>
      <givenName>Angela</givenName>
      <familyName>Mally</familyName>
      <affiliation>Univ Wurzburg, Dept Toxicol, D-97070 Wurzburg, Germany</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Assessment Of Global And Gene-Specific Dna Methylation In Rat Liver And Kidney In Response To Non-Genotoxic Carcinogen Exposure</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2015</publicationYear>
  <dates>
    <date dateType="Issued">2015-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/82301</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.taap.2015.09.023</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Altered expression of tumor suppressor genes and oncogenes, which is regulated in part at the level of DNA methylation, is an important event involved in non-genotoxic carcinogenesis. This may serve as a marker for early detection of non-genotoxic carcinogens. Therefore, we evaluated the effects of non-genotoxic hepatocarcinogens, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), hexachlorobenzene (HCB), methapyrilene (MPY) and male rat kidney carcinogens, d-limonene, p-dichlorobenzene (DCB), chloroform and ochratoxin A (OTA) on global and CpG island promoter methylation in their respective target tissues in rats. No significant dose-related effects on global DNA hypomethylation were observed in tissues of rats compared to vehicle controls using LC-MS/MS in response to short-term non-genotoxic carcinogen exposure. Initial experiments investigating gene-specific methylation using methylation-specific PCR and bisulfite sequencing, revealed partial methylation of p16 in the liver of rats treated with HCB and TCDD. However, no treatment related effects on the methylation status of Cx32, e-cadherin, VHL, c-myc, Igfbp2, and p15 were observed. We therefore applied genome-wide DNA methylation analysis using methylated DNA immunoprecipitation combined with microarrays to identify alterations in gene-specific methylation. Under the conditions of our study, some genes were differentially methylated in response to MPY and TCDD, whereas d-limonene, DCB and chloroform did not induce any methylation changes. 90-day OTA treatment revealed enrichment of several categories of genes important in protein kinase activity and mTOR cell signaling process which are related to OTA nephrocarcinogenicity. (C) 2015 Elsevier Inc. All rights reserved.</description>
  </descriptions>
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