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Alcay, Selim; Cakmak, Selvinar; Cakmak, Ibrahim; Mulkpinar, Emine; Toker, Mehmed Berk; Ustuner, Burcu; Sen, Hasan; Nur, Zekariya
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/76191</identifier> <creators> <creator> <creatorName>Alcay, Selim</creatorName> <givenName>Selim</givenName> <familyName>Alcay</familyName> <affiliation>Uludag Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Cakmak, Selvinar</creatorName> <givenName>Selvinar</givenName> <familyName>Cakmak</familyName> <affiliation>Uludag Univ, Beekeeping Dev Applicat & Res Ctr, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Cakmak, Ibrahim</creatorName> <givenName>Ibrahim</givenName> <familyName>Cakmak</familyName> <affiliation>Uludag Univ, Beekeeping Dev Applicat & Res Ctr, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Mulkpinar, Emine</creatorName> <givenName>Emine</givenName> <familyName>Mulkpinar</familyName> <affiliation>Uludag Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Toker, Mehmed Berk</creatorName> <givenName>Mehmed Berk</givenName> <familyName>Toker</familyName> <affiliation>Uludag Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Ustuner, Burcu</creatorName> <givenName>Burcu</givenName> <familyName>Ustuner</familyName> <affiliation>Uludag Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Sen, Hasan</creatorName> <givenName>Hasan</givenName> <familyName>Sen</familyName> <affiliation>Uludag Univ, Beekeeping Dev Applicat & Res Ctr, TR-16059 Bursa, Turkey</affiliation> </creator> <creator> <creatorName>Nur, Zekariya</creatorName> <givenName>Zekariya</givenName> <familyName>Nur</familyName> <affiliation>Uludag Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, TR-16059 Bursa, Turkey</affiliation> </creator> </creators> <titles> <title>Drone Semen Cryopreservation With Protein Supplemented Tl-Hepes Based Extender</title> </titles> <publisher>Aperta</publisher> <publicationYear>2019</publicationYear> <dates> <date dateType="Issued">2019-01-01</date> </dates> <resourceType resourceTypeGeneral="Text">Journal article</resourceType> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/76191</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.9775/kvfd.2018.21311</relatedIdentifier> </relatedIdentifiers> <rightsList> <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract">The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10(6) spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P&lt; 0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P&lt; 0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.</description> </descriptions> </resource>
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