1 Ocak 2019 Dergi makalesi Açık Erişim

Arabaci, Nihan; Arikan, Burhan

Citation Style Language JSON

{
  "DOI": "10.1080/10826068.2018.1550655", 
  "abstract": "An amylopullulanase was produced by Geobacillus thermoleovorans NP1. The optimum enzyme production occurred at 45 degrees C and pH 7.0 (12 hr). NP1 amylopullulanase (ApuNP1) exhibited the maximal activity at 50 degrees C and pH 6.0 and was stable between 30-50 degrees C, and pH 3.0-12.0 for 24 hr. The enzyme showed two bands with molecular weights of 112 and 107 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The amylopullulanase retained 100% of its activity in the presence of 10 mM of Ca2+, Ba2+, Zn2+, Mg2+, Cu2+, EDTA, and PMSF. While the enzyme showed resistance to 5% of TritonX-100, Tween 20, and Tween 80, the activity was inhibited by 5% -mercaptoethanol and H2O2. While the hydrolysis products of pullulan were maltose, maltotriose, and maltodextrin, the starch was hydrolyzed to maltose, maltotriose, and maltodextrin units. This shows that NP1 pullulanase is a type II pullulanase (amylopullulanase). After the liquefaction assay, 12% glucose content was measured with a refractometer in the presence of 20% starch. According to the wash performance tests, the mixture of ApuNP1 and 1% detergent removed almost all of the stains. This novel thermo-acidic amylopullulanase has a potency to be used in detergent, starch, food, baking, textile, and cosmetic industries.", 
  "author": [
    {
      "family": "Arabaci", 
      "given": " Nihan"
    }, 
    {
      "family": "Arikan", 
      "given": " Burhan"
    }
  ], 
  "container_title": "PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY", 
  "id": "75147", 
  "issue": "2", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "page": "127-135", 
  "title": "An amylopullulanase (ApuNP1) from Geobacillus thermoleovorans NP1: biochemical characterization and its potential industrial applications", 
  "type": "article-journal", 
  "volume": "49"
}