1 Ocak 2019 Dergi makalesi Açık Erişim

Erenler, Ayse Sebnem; Geckil, Hikmet; Karabulut, Aysun Bay; Akpolat, Nusret; Sevimli, Resit; Ulke, Esra; Aliyeva, Aygun

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        "affiliation": "Inonu Univ, Dept Biol, TR-44280 Malatya, Turkey", 
        "name": "Erenler, Ayse Sebnem"
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      {
        "affiliation": "Inonu Univ, Dept Mol Biol & Genet, TR-44280 Malatya, Turkey", 
        "name": "Geckil, Hikmet"
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      {
        "affiliation": "Turgut Ozal Univ, TR-44420 Malatya, Turkey", 
        "name": "Karabulut, Aysun Bay"
      }, 
      {
        "affiliation": "Inonu Univ, Dept Surg Med Sci, TR-44280 Malatya, Turkey", 
        "name": "Akpolat, Nusret"
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      {
        "affiliation": "Inonu Univ, Dept Surg Med Sci, TR-44280 Malatya, Turkey", 
        "name": "Sevimli, Resit"
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      {
        "affiliation": "Inonu Univ, Dept Biol, TR-44280 Malatya, Turkey", 
        "name": "Ulke, Esra"
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        "affiliation": "Inonu Univ, Dept Med Sci, TR-44280 Malatya, Turkey", 
        "name": "Aliyeva, Aygun"
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    "description": "This study focused on a new plasmid and new recombinant strain developed for the production of microbial chondroitin sulfate a new and limited area of study the strategies we applied for the production of microbial chondroitin sulfate, and the possible contributions of this study to published research literature. In this study, pETM6-PACF, which carries the genes responsible for capsular chondroitin synthesis [kfA, kfoC, kfoF] was used as the basic plasmid. The Vitreoscilla hemoglobin gene region was transformed into this basic plasmid and the common expression of both gene groups was added to research literature for the first time. This plasmid was transferred to non-pathogenic E. coil (C2987) to produce a completely new chondroitin source specific to this study. Following the transformation by chondroitin synthesis, and the subsequent microbial production of chondroitin by the application of purification protocols, microbial chondroitin sulfate was produced in sulfate form. Consequently, in comparison to published literature, a product with a low molecular weight value of 269 Daltons was developed. This product, which has significant potential drug potency, can be used in many different areas as a novel and unique biomedical product.", 
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    "journal": {
      "issue": "12", 
      "pages": "1745-1754", 
      "title": "SCIENCE OF ADVANCED MATERIALS", 
      "volume": "11"
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    "publication_date": "2019-01-01", 
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      "title": "Dergi makalesi", 
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    "title": "Cloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production"
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