1 Ocak 2019 Dergi makalesi Açık Erişim

Avci, Edibe; Akkaya-Ulum, Yeliz Z.; Yoyen-Ermis, Digdem; Esendagli, Gunes; Balci-Peynircioglu, Banu

DataCite XML

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/73191</identifier>
  <creators>
    <creator>
      <creatorName>Avci, Edibe</creatorName>
      <givenName>Edibe</givenName>
      <familyName>Avci</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Med Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Akkaya-Ulum, Yeliz Z.</creatorName>
      <givenName>Yeliz Z.</givenName>
      <familyName>Akkaya-Ulum</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Med Biol, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yoyen-Ermis, Digdem</creatorName>
      <givenName>Digdem</givenName>
      <familyName>Yoyen-Ermis</familyName>
    </creator>
    <creator>
      <creatorName>Esendagli, Gunes</creatorName>
      <givenName>Gunes</givenName>
      <familyName>Esendagli</familyName>
      <affiliation>Hacettepe Univ, Dept Basic Oncol, Canc Inst, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Balci-Peynircioglu, Banu</creatorName>
      <givenName>Banu</givenName>
      <familyName>Balci-Peynircioglu</familyName>
      <affiliation>Hacettepe Univ, Fac Med, Dept Med Biol, Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>A Method For High-Purity Isolation Of Neutrophil Granulocytes For Functional Cell Migration Assays</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/73191</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1515/tjb-2019-0089</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Background: Neutrophil-mediated killing of pathogens is one of the most significant functions of the primary defense of the host. Neutrophil activity and migration play a key role in inflammatory conditions. To gain insights into the interactions between neutrophils and neutrophil migration-related disorders, a large number of sophisticated methods have been developed. The technical limitations of isolating highly purified neutrophil populations, minimizing both cell death and activation during the isolation process, and the short lifespan of neutrophils present challenges for studying specific functions of neutrophils in vitro. In this study, we aimed to evaluate a separation medium-based density gradient method to obtain highly purified neutrophil populations and combined this protocol with a model for studying neutrophil migration in-vitro.</description>
  </descriptions>
</resource>